Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

GTP binding proteins: a key role in cellular communication.

J Bockaert1, V Homburger, B Rouot

  • 1Centre CNRS-INSERM de Pharmacologie-Endocrinologie, Montpellier, France.

Biochimie
|April 1, 1987
PubMed
Summary

Guanine nucleotide-binding proteins (G proteins) are crucial for cell signaling, coupling receptors to cellular effectors. Research details the structure and function of Gs, Gi, and transducin, highlighting their roles in hormonal and sensory pathways.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chitinase 3-like proteins as diagnostic and prognostic biomarkers of multiple sclerosis.

Multiple sclerosis (Houndmills, Basingstoke, England)·2015
Same author

The nucleus accumbens 5-HTR₄-CART pathway ties anorexia to hyperactivity.

Translational psychiatry·2012
Same author

Metabotropic glutamate receptors: from the workbench to the bedside.

Neuropharmacology·2010
Same author

?(1)- and ?(2)-adrenergic receptors in rat corpus striatum.

Neurochemistry international·2010
Same author

Physical interaction between the serotonin transporter and neuronal nitric oxide synthase underlies reciprocal modulation of their activity.

Proceedings of the National Academy of Sciences of the United States of America·2007
Same author

[The Lemierre syndrome: a complicated oropharyngeal infection].

Acta clinica Belgica·2005

Area of Science:

  • Cellular biology
  • Molecular biology
  • Biochemistry

Background:

  • Guanine nucleotide-binding proteins (G proteins) are key mediators of signal transduction in eukaryotic cells.
  • The discovery of Gs protein, essential for hormonal stimulation of adenylate cyclase, marked a significant advancement.
  • G proteins link cell surface receptors to intracellular effectors, regulating diverse cellular processes.

Purpose of the Study:

  • To elucidate the structure and function of various G proteins involved in cellular signaling.
  • To detail the purification and characterization of Gs, Gi, transducin, Go, and Gp proteins.
  • To explore the common structural design and functional similarities among different G proteins.

Main Methods:

  • Protein purification of G proteins from various tissues and cell types.

Related Experiment Videos

  • Biochemical characterization of G protein subunits (alpha, beta, gamma).
  • Analysis of ADP ribosylation patterns by bacterial toxins (cholera toxin, pertussis toxin).

    • Main Results:

    • Gs, Gi, and transducin were purified and their roles in adenylate cyclase and phosphodiesterase regulation identified.
    • Go and Gp proteins were isolated, with Go primarily found in nervous tissues.
    • All characterized G proteins share a common heterotrimeric structure (alpha, beta, gamma subunits) with conserved domains, particularly in the GTP-binding site.

    Conclusions:

    • G proteins represent a conserved family of signal transducers with a common structural framework.
    • The alpha subunits exhibit variability but share homology with other GTP-binding proteins and are targets for bacterial toxin modification.
    • Understanding G protein diversity and function is critical for deciphering complex cellular communication networks.