Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Scanning Electron Microscopy

Scanning Electron Microscopy

A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...

Leaky Scanning

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...

Voltammetric Techniques: Linear-Scan (E vs Time)

Voltammetric Techniques: Linear-Scan (E vs Time)

Polarography is a classical voltammetric technique used to analyze electrochemical reactions. This method applies a linear potential sweep to a dropping mercury electrode (DME), and the resulting current is measured. A dropping mercury electrode is commonly used as the working electrode in polarography. It consists of a capillary tube filled with mercury, where the tiny droplet forms at the tip. This droplet continuously drops from the capillary, creating a new electrode surface for each...

Radiological Investigation II: MRI and Ventilation Perfusion Scan

Radiological Investigation II: MRI and Ventilation Perfusion Scan

Description
Magnetic Resonance Imaging (MRI) and Ventilation Perfusion Scans are two radiological investigations that offer detailed diagnostic images of the body, particularly lung structures.
MRI
MRI uses magnetic fields and radiofrequency signals to distinguish between normal and abnormal tissues. This technology provides a more detailed diagnostic image than CT scans, enabling it to characterize pulmonary nodules, stage bronchogenic carcinoma, and evaluate inflammatory activity in...

Immunofluorescence Microscopy

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.

Radiological Investigation III: Pulmonary Angiogram and PET Scan

Radiological Investigation III: Pulmonary Angiogram and PET Scan

Radiological investigations are paramount in the diagnosis and management of various pulmonary diseases. Two essential investigations are the Pulmonary Angiogram and the Positron Emission Tomography (PET) Scan.
Pulmonary Angiogram
A Pulmonary Angiogram is an invasive procedure involving injecting a contrast medium through a catheter threaded into the pulmonary artery or the right side of the heart to visualize the pulmonary vasculature. Computed Tomography (CT) scans have mainly replaced this...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Author Correction: Magnetic memory and spontaneous vortices in a van der Waals superconductor.

Nature·2023

Same author

Magnetic memory and spontaneous vortices in a van der Waals superconductor.

Nature·2022

Same author

Anomalous transport in high-mobility superconducting SrTiO<sub>3</sub> thin films.

Science advances·2022

Same author

Fabrication of Magnetic Platforms for Micron-Scale Organization of Interconnected Neurons.

Journal of visualized experiments : JoVE·2021

Same author

Non-universal current flow near the metal-insulator transition in an oxide interface.

Nature communications·2021

Same author

Sensitive Readout for Microfluidic High-Throughput Applications using Scanning SQUID Microscopy.

Scientific reports·2020

Search research articles

Related Experiment Video

Updated: Jan 24, 2026

Scanning SQUID Study of Vortex Manipulation by Local Contact

Scanning SQUID Study of Vortex Manipulation by Local Contact

Published on: February 1, 2017

Scanning SQUID microscopy in a cryogen-free cooler.

Yishai Shperber¹, Naor Vardi¹, Eylon Persky¹

¹Department of Physics and Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat-Gan, Israel.

The Review of Scientific Instruments

|June 3, 2019

Summary

This study demonstrates successful scanning superconducting quantum interference device (SQUID) microscopy in a cryogen-free system. The cryogen-free SQUID microscope achieves performance comparable to traditional liquid helium systems.

More Related Videos

Optimizing Sample Preparation for Cryogenic Electron Microscopy

Optimizing Sample Preparation for Cryogenic Electron Microscopy

Published on: April 11, 2025

A Method for Extracting Pigments from Squid Doryteuthis pealeii

A Method for Extracting Pigments from Squid Doryteuthis pealeii

Published on: November 9, 2016

Related Experiment Videos

Last Updated: Jan 24, 2026

Scanning SQUID Study of Vortex Manipulation by Local Contact

Scanning SQUID Study of Vortex Manipulation by Local Contact

Published on: February 1, 2017

Optimizing Sample Preparation for Cryogenic Electron Microscopy

Optimizing Sample Preparation for Cryogenic Electron Microscopy

Published on: April 11, 2025

A Method for Extracting Pigments from Squid Doryteuthis pealeii

A Method for Extracting Pigments from Squid Doryteuthis pealeii

Published on: November 9, 2016

Area of Science:

Condensed Matter Physics
Materials Science
Quantum Technologies

Background:

Scanning superconducting quantum interference device (SQUID) microscopy is vital for probing magnetic signals at surfaces and interfaces.
Traditional SQUID microscopy relies on liquid helium for cryogenic temperatures, posing logistical and cost challenges.
Transitioning to cryogen-free systems is desirable but hindered by increased electrical noise and vibrations.

Purpose of the Study:

To report the successful operation of a scanning SQUID microscope in a cryogen-free cooling system.
To evaluate the performance and capabilities of a cryogen-free SQUID microscope.
To address the challenges associated with cryogen-free SQUID operation.

Main Methods:

Utilized a modified Montana Instruments cryogen-free cooler achieving a base temperature of 4.3 K.
Performed scanning SQUID measurements to assess flux noise performance.
Correlated sensor-sample vibrations with cryocooler operational frequencies.
Demonstrated various SQUID operation modes.

Main Results:

Achieved successful scanning SQUID microscopy operation in a cryogen-free environment.
Demonstrated flux noise performance comparable to liquid helium-based (wet) systems.
Identified and correlated sensor-sample vibrations to cryocooler frequencies.
Successfully operated in multiple SQUID modes, including magnetic field mapping, local susceptibility measurements, and current flow mapping.

Conclusions:

Cryogen-free SQUID microscopy is feasible and offers performance comparable to conventional systems.
The developed system overcomes challenges of noise and vibration in cryogen-free operation.
This advancement enables wider accessibility and application of SQUID microscopy in various scientific fields.