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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
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CRISPR/Cas Multiplexed Biosensing: A Challenge or an Insurmountable Obstacle?

Yi Li1, Linyang Liu1, Guozhen Liu2

  • 1Graduate School of Biomedical Engineering, ARC Centre of Excellence in Nanoscale Biophotonics, Faculty of Engineering, University of New South Wales, Sydney, NSW 2052, Australia; Australian Centre for NanoMedicine, University of New South Wales, Sydney, NSW 2052, Australia.

Trends in Biotechnology
|June 3, 2019
PubMed
Summary

Multiplex detection for molecular diagnostics is challenging but achievable using CRISPR/Cas-based biosensing. Strategic planning is key to realizing the full potential of CRISPR multiplexed diagnostics.

Keywords:
CRISPR/Casbiosensingdiagnosticsmultiplexnucleic acid detection

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Area of Science:

  • Molecular Diagnostics
  • Biosensing Technologies
  • Gene Editing Tools

Background:

  • Multiplex detection, the simultaneous identification of multiple analytes, remains a significant challenge in molecular diagnostics.
  • CRISPR/Cas systems offer a promising platform for developing sensitive and specific biosensors.
  • Previous research has shown the potential of CRISPR/Cas-based biosensing for multiplexed assays, though widespread application is still limited.

Discussion:

  • CRISPR/Cas multiplexed biosensing presents a realistic, albeit complex, challenge rather than an insurmountable barrier.
  • Recent successful applications highlight the feasibility and growing capabilities of this technology.
  • Careful strategic considerations are necessary to overcome existing limitations and optimize performance.

Key Insights:

  • CRISPR/Cas technology is advancing multiplex detection capabilities in molecular diagnostics.
  • Successful implementations demonstrate the viability of CRISPR-based multiplexed biosensing.
  • Strategic development is crucial for unlocking the full diagnostic potential.

Outlook:

  • Further research and strategic planning will enhance CRISPR/Cas multiplexed biosensing for broader clinical use.
  • This technology holds promise for revolutionizing molecular diagnostics through efficient multiplexed assays.
  • Continued innovation is expected to address current challenges and expand applications.