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Hydrogen sulfide impacts on inflammation-induced adipocyte dysfunction.

Ferran Comas1, Jèssica Latorre1, Olaf Cussó2

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Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association
|June 3, 2019
PubMed
Summary

Hydrogen sulfide (H₂S) plays a dual role in inflammation and adipogenesis. Slow-releasing H₂S donors improve adipocyte differentiation, especially under inflammatory conditions, with effects dependent on dose and exposure time.

Keywords:
3T3-L13T3-L1 cell differentiationAdipocyteGYY4137Hydrogen sulfideInflammationNa(2)S

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Metabolism

Background:

  • Hydrogen sulfide (H₂S) exhibits a dual role in inflammation.
  • Recent studies suggest H₂S influences adipogenesis in 3T3-L1 cells.

Purpose of the Study:

  • To investigate the effects of H₂S on adipocyte differentiation and inflammation.
  • To determine how H₂S concentration, donor type, and exposure time impact adipogenesis.

Main Methods:

  • Utilized 3T3-L1 cells for adipocyte differentiation studies.
  • Administered H₂S donors (GYY4137, Na₂S) and an inhibitor (DL-Propargyl Glycine, PPG).
  • Assessed adipogenic gene expression and insulin signaling markers under normal and inflammatory conditions.

Main Results:

  • Endogenous H₂S levels increased during 3T3-L1 differentiation; inhibition impaired this process.
  • Specific timing of H₂S donor administration influenced adipogenic gene expression, with excessive or continuous exposure showing negative effects.
  • Slow-releasing H₂S (GYY4137) attenuated inflammation-induced negative effects on adipogenesis and insulin signaling, while Na₂S exacerbated them.

Conclusions:

  • H₂S exerts pro-adipogenic effects that are dependent on the dose, donor type, and duration of exposure.
  • Slow-releasing H₂S donors show potential in improving adipocyte differentiation in inflammatory contexts.
  • The dual role of H₂S in adipogenesis and inflammation is complex and context-dependent.