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Methodology to Test Control Agents and Insecticides Against the Coffee Berry Borer Hypothenemus hampei

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Targeted proteomic response to coffee consumption.

Alan Kuang¹, Iris Erlund², Christian Herder^3,4,5

¹Department of Preventive Medicine, Northwestern University Feinberg School of Medicine, 680 North Lake Shore Drive, Suite 1400, Chicago, IL, 60611, USA.

European Journal of Nutrition

|June 3, 2019

Summary

This summary is machine-generated.

Coffee consumption significantly increased two novel neurological proteins, carboxypeptidase M (CPM) and neutral ceramidase (N-CDase). These findings suggest new biological pathways through which coffee may impact health.

Keywords:

Biomarkers Caffeine Coffee Proteomics Trial

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Area of Science:

Biochemistry
Proteomics
Human Health

Background:

Coffee is a globally consumed beverage with diverse health implications.
The precise biological mechanisms underlying coffee's health effects remain largely unknown.
Understanding coffee's impact on specific molecular pathways is crucial for elucidating its health benefits.

Purpose of the Study:

To investigate the effect of varying coffee consumption levels on candidate proteins.
To identify proteins involved in cardiovascular, immuno-oncological, and neurological pathways that respond to coffee intake.
To explore novel biomarkers associated with coffee consumption.

Main Methods:

A single-blinded, three-stage clinical trial involving 47 habitual coffee consumers.
Participants abstained from coffee for one month, then consumed 4 cups/day, followed by 8 cups/day.
Serum samples were analyzed using proximity extension assays to measure 247 proteins; repeated measures ANOVA was used for statistical analysis.

Main Results:

Two neurology-related proteins, carboxypeptidase M (CPM) and neutral ceramidase (N-CDase or ASAH2), showed significant increases post-coffee consumption (P < 0.05, Q < 0.05).
An additional 46 proteins were nominally associated with coffee intake (P < 0.05).
Of the associated proteins, 41 increased with coffee intake, with significant representation in neurological, cardiovascular, and immuno-oncological pathways.

Conclusions:

Coffee consumption led to increased levels of CPM and N-CDase.
These proteins represent novel biological markers of coffee intake.
Further research is warranted to explore the functional significance of these coffee-responsive proteins.