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Updated: Jan 24, 2026

Bacterial Inner-membrane Display for Screening a Library of Antibody Fragments
Published on: October 15, 2016
Synthesis and screening of bead-displayed combinatorial libraries
Todd M Doran1, Paige Dickson2, John Maina Ndungu2
1Department of Medicinal Chemistry and the Institute for Translational Neuroscience, University of Minnesota, Minneapolis, MN, United States.
Abstract:
The development of faster and less expensive methods to discover bioactive small molecules remains a high priority in chemical biology. This article discusses one alternative to traditional high-throughput screening: the synthesis and screening of one bead one compound (OBOC) libraries. Protocols are provided to create and screen libraries of peptoid displayed on TentaGel beads, which is a cheap and relatively straightforward process for the identification of selective protein ligands. However, peptoids bind to proteins with modest affinity in most cases. Therefore, we also describe protocols to create libraries of stiffer oligomers called PICCOs (peptoid-inspired, conformationally constrained oligomers) that have proven to be a superior source of high affinity ligands.
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