Effects of Alpha-tocopherol Antioxidant on Dentin-composite Microtensile Bond Strength after Sodium Perborate...

It has been reported that bond strength can be reversed to prebleached levels with the application of 10% alpha-tocopherol in a 2-hour time frame or by delaying bonding for 2 weeks. This study evaluated the effectiveness of a 5-minute application of 20% alpha-tocopherol to reverse the deleterious effects of nonvital bleaching on consequent bond strength. Thirty third molars were assigned to the following 3 groups: unbleached, bleached, and bleached followed by treatment with alpha-tocopherol. The bleached groups were exposed to sodium perborate (2 g/mL) for 7 days. The postbleach treatment group was subsequently treated with 20% alpha-tocopherol for 5 minutes, and then all groups were restored with composite resin. After 24 hours of storage at 37°C and 100% humidity, restored tooth specimens were sectioned into 1-mm² dentin-composite beams. Six beams from each tooth were subjected to microtensile bond strength testing. Representative beams were further evaluated with Raman microspectroscopy and scanning electron microscopy. The mean bond strength values (MPa) for each group were as follows: unbleached control group = 26.2, bleached control group = 20.3, and post-bleach treatment group = 18.5. A 1-factor analysis of variance and Tukey post hoc test (α = 0.05) indicated that bleaching had a detrimental effect on bond strength and that short-term alpha-tocopherol treatments did not improve postbleach bond strength. Raman microspectroscopy and scanning electron microscopy revealed no noted improvement for the post-bleach treatment group.The application of 20% alpha-tocopherol in a clinically relevant time frame was not effective in counteracting the deleterious effect of bleaching on bond strength. Bonding procedures should be delayed after tooth bleaching.