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Published on: January 2, 2013
Metformin protects red blood cells against rotenone induced oxidative stress and cytotoxicity
Shambhoo Sharan Tripathi1, Abhishek Kumar Singh1, Farhan Akhtar1
1Department of Biochemistry, University of Allahabad, Allahabad, India.
Context:
The anti-diabetic medicine metformin has been reported as an anti-ageing drug candidate as it mimics the benefits of caloric restriction and reduces ageing-related oxidative stress in various experimental organisms.
Objective:
We investigated the possible anti-oxidative role of metformin against rotenone-induced oxidative stress and cytotoxicity in erythrocytes of Wistar rats. Rotenone is a well-known inducer of oxidative stress which leads to a cellular redox imbalance.
Materials And Methods:
We have co-exposed the experimental rats with rotenone (2.5 mg/kg, i.p.) and metformin (300 mg/kg, orally) for 30 days to investigate the protective effects of metformin on various rotenone-induced impaired oxidative stress biomarkers in rat erythrocytes.
Results:
We found that a significant alleviation in the levels of rotenone-induced pro-oxidant and anti-oxidant markers following exposure of metformin.
Discussion And Conclusions:
Our findings suggest that metformin supplementation shows a protective role in against rotenone-induced redox imbalance and cytotoxicity in rat erythrocytes.
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