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Updated: Jan 24, 2026

Whisker-signaled Eyeblink Classical Conditioning in Head-fixed Mice
Published on: March 30, 2016
Single-Unit Extracellular Recording from the Cerebellum During Eyeblink Conditioning in Head-Fixed Mice
Abstract:
This chapter presents a method for performing in vivo single-unit extracellular recordings and optogenetics during an associative, cerebellum-dependent learning task in head-fixed mice. The method uses a cylindrical treadmill system that reduces stress in the mice by allowing them to walk freely, yet it provides enough stability to maintain single-unit isolation of neurons for tens of minutes to hours. Using this system, we have investigated sensorimotor coding in the cerebellum while mice perform learned skilled movements.
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