Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Biofilms

Biofilms

Biofilms are complex communities of microorganisms encased in a self-produced extracellular polysaccharide matrix attached to surfaces. These microbial consortia can include single or multiple species, providing enhanced survival benefits by forming organized, multilayered structures.The formation of biofilms occurs through four key stages: attachment, colonization, development, and dispersal.During attachment, free-swimming planktonic cells adhere to a surface, often facilitated by...

What is Genetic Engineering?

What is Genetic Engineering?

Fixed Action Patterns

Fixed Action Patterns

A fixed action pattern (FAP) is a specific, hard-wired sequence of behaviors that occurs in response to an external stimulus, called a sign stimulus. The behavior is “fixed” because it is essentially unchangeable—proceeding similarly across individuals of a species every time it occurs.

Dimensional Analysis

Dimensional Analysis

Dimensional analysis, also known as the factor label method, is a versatile approach for mathematical operations. The main principle behind this approach is: the units of quantities must be subjected to the same mathematical operations as their associated numbers. This method can be applied to computations ranging from simple unit conversions to more complex and multi-step calculations involving several different quantities and their units.
Conversion Factors and Dimensional Analysis
The unit...

Dimensional Analysis

Dimensional Analysis

Dimensional analysis is a valuable technique in fluid mechanics for simplifying complex problems by reducing them into dimensionless groups. These groups capture the essential relationships between the variables involved, allowing researchers and engineers to analyze fluid flow without dealing with each variable individually. This approach reduces the number of independent variables, allowing for easier analysis and better understanding of physical phenomena.
In fluid mechanics, dimensional...

Patterns of Fever

Patterns of Fever

Before understanding the types and patterns of fever, it is essential to know its phases.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Prenucleation Clusters in Shell Formation of Photoluminescent Core/Shell Quantum Dots of Heavy-Metal-Free ZnTeSe/ZnSe.

Small (Weinheim an der Bergstrasse, Germany)·2026

Same author

Multi-View Causal Feature Selection.

IEEE transactions on pattern analysis and machine intelligence·2026

Same author

Spore inoculum size impacts substrate degradation and sporulation but not the secretome during colonization of whole yellow pea (Pisum sativum) by Aspergillus oryzae.

International journal of food microbiology·2026

Same author

Reverse prediction of carbohydrate esterase polysaccharide targets.

Biotechnology for biofuels and bioproducts·2026

Same author

A Hadza-enriched <i>Prevotella/Segatella</i> xyloglucanase shows sequence conservation and functional specialization.

Gut microbes reports·2026

Same author

3D-Bioprinted Marine Bacteria for the Degradation of Polyhydroxybutyrate Bioplastics.

ACS applied polymer materials·2026

Search research articles

Related Experiment Video

Updated: Jan 24, 2026

Three-dimensional Patterning of Engineered Biofilms with a Do-it-yourself Bioprinter

Three-dimensional Patterning of Engineered Biofilms with a Do-it-yourself Bioprinter

Published on: May 16, 2019

Three-dimensional Patterning of Engineered Biofilms with a Do-it-yourself Bioprinter.

Ewa M Spiesz¹, Kui Yu¹, Benjamin A E Lehner¹

¹Department of Bionanoscience & Kavli Institute of Nanoscience, Delft University of Technology.

Journal of Visualized Experiments : Jove

|June 4, 2019

Summary

Researchers developed a novel 3D bioprinter using bacterial ink for creating biofilms. This technology enables precise spatial control of biofilms for applications in antibiotic resistance and water purification.

More Related Videos

Microfluidic Bioprinting for Engineering Vascularized Tissues and Organoids

Microfluidic Bioprinting for Engineering Vascularized Tissues and Organoids

Published on: August 11, 2017

High-resolution Patterned Biofilm Deposition Using pDawn-Ag43

High-resolution Patterned Biofilm Deposition Using pDawn-Ag43

Published on: October 23, 2018

Related Experiment Videos

Last Updated: Jan 24, 2026

Three-dimensional Patterning of Engineered Biofilms with a Do-it-yourself Bioprinter

Three-dimensional Patterning of Engineered Biofilms with a Do-it-yourself Bioprinter

Published on: May 16, 2019

Microfluidic Bioprinting for Engineering Vascularized Tissues and Organoids

Microfluidic Bioprinting for Engineering Vascularized Tissues and Organoids

Published on: August 11, 2017

High-resolution Patterned Biofilm Deposition Using pDawn-Ag43

High-resolution Patterned Biofilm Deposition Using pDawn-Ag43

Published on: October 23, 2018

Area of Science:

Microbiology
Biotechnology
Materials Science

Background:

Biofilms, bacterial aggregates in a matrix, exhibit enhanced antibiotic resistance.
This resistance poses health risks but also offers environmental benefits, like water purification.
Developing controllable biofilm creation methods is crucial for therapeutics and biomaterials.

Purpose of the Study:

To describe the construction of an efficient, low-cost 3D bioprinter for biofilm fabrication.
To present a novel method for creating spatially-controlled biofilms using engineered bacteria.
To highlight the potential applications of this technology in various scientific and industrial fields.

Main Methods:

Adapted a commercial 3D printer by replacing the extruder with a bio-ink dispenser and syringe pump.
Developed a bio-ink by suspending engineered Escherichia coli in alginate.
Utilized a calcium-containing substrate and an inducer chemical to promote biofilm formation and protein expression.

Main Results:

Successfully fabricated a 3D bioprinter capable of continuous bio-ink flow.
Demonstrated the ability to print discrete layers of biofilms in various spatial patterns.
Engineered bacteria in alginate formed solid structures upon contact with calcium, facilitated by an inducer.

Conclusions:

The developed 3D bioprinter offers a reproducible and engineerable method for biofilm creation.
Spatially-controlled biofilms can serve as valuable model systems for research.
This technology has broad societal impact potential in areas such as antibiotic resistance prevention and water purification.