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Updated: Jan 24, 2026

High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue
Published on: September 30, 2022
Spectroscopic setup for submicrometer-resolution mapping of low-signal absorption and luminescence using photothermal
Abstract:
A spectroscopic setup that enables the mapping of absorption and photoluminescence with submicrometer spatial resolution and high sensitivity is described. A photothermal heterodyne imaging pump/probe technique is employed for absorption mapping, and low-signal, spatially resolved photoluminescence is recorded using photon counting. High-spatial-resolution mapping is accomplished by using high-numerical-aperture microscope objective focusing laser beams (pump and probe) into a submicrometer spot and raster-scanning sample mounted on the nanopositioning translation stage. Performance of the setup is illustrated by mapping absorption and luminescence of the hafnium oxide film with embedded hafnium nanoparticles and multilayer dielectric grating. In both cases, submicrometer spatial resolution is demonstrated, and possible physical mechanisms leading to image contrast on a submicrometer scale are discussed.
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