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Induction of c-fos proto-oncogene by a chemotactic peptide in human peripheral granulocytes
FEBS Letters
|October 5, 1987
Abstract:
The chemotactic peptide, fMet-Leu-Phe (fMLP), induced proto-oncogene c-fos mRNA in purified human peripheral granulocytes. The induction was transient, and was inhibited by pertussis toxin or by an inhibitor of protein kinase C. These results suggest that activation of a guanine nucleotide-binding protein and of protein kinase C is involved in c-fos induction in granulocytes.
Insights
The peptide fMet-Leu-Phe (fMLP) triggers c-fos mRNA in human granulocytes. This process involves guanine nucleotide-binding proteins and protein kinase C, and is blocked by pertussis toxin.
Area of Science:
- Immunology
- Molecular Biology
- Cell Signaling
Background:
- Proto-oncogene c-fos plays a critical role in cellular responses.
- Granulocytes are key immune cells involved in inflammation and host defense.
- Chemotactic peptides initiate signaling cascades within immune cells.
Purpose of the Study:
- To investigate the induction of proto-oncogene c-fos mRNA by fMet-Leu-Phe (fMLP) in human granulocytes.
- To identify the signaling pathways involved in fMLP-induced c-fos expression.
Main Methods:
- Purification of human peripheral granulocytes.
- Treatment with fMet-Leu-Phe (fMLP).
- Analysis of c-fos mRNA levels.
- Inhibition studies using pertussis toxin and protein kinase C inhibitors.
Main Results:
- fMet-Leu-Phe (fMLP) induced a transient expression of c-fos mRNA in human granulocytes.
- Pertussis toxin inhibited fMLP-induced c-fos mRNA expression.
- Inhibition of protein kinase C also blocked c-fos induction.
Conclusions:
- Activation of guanine nucleotide-binding proteins is implicated in fMLP-mediated c-fos induction.
- Protein kinase C signaling is essential for the transient expression of c-fos mRNA.
- These findings elucidate key molecular mechanisms in granulocyte activation.