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A novel long non-coding RNA TONSL-AS1 regulates progression of gastric cancer via activating TONSL

Peng Wang1, Xi Yang2, Liang Zhao3

  • 1Department of General Surgery, The Affiliated Jiangsu Shengze Hospital of Nanjing Medical University. No.1399, Shichang Road, Shengze Town, Suzhou City, Jiangsu, China; Department of General Surgery, The Second Hospital of Nanjing, Nanjing University of Chinese Medicine. No.1-1, Zhongfu Road, Nanjing, Jiangsu, China.

Experimental Cell Research
|June 4, 2019
PubMed

Insights

A novel long non-coding RNA (lncRNA), TONSL-AS1, acts as a tumor suppressor in gastric cancer. It inhibits cell growth and metastasis, offering a potential new therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long non-coding RNAs (lncRNAs) are implicated in various cancers.
  • The specific roles of lncRNAs in gastric cancer remain largely undefined.
  • Identifying novel regulatory mechanisms is crucial for gastric cancer treatment.

Purpose of the Study:

  • To investigate the function of a novel lncRNA, TONSL-AS1, in gastric cancer.
  • To elucidate the molecular mechanism underlying TONSL-AS1's role in gastric cancer.
  • To assess TONSL-AS1 as a potential therapeutic target for gastric cancer.

Main Methods:

  • Quantitative real-time PCR to assess TONSL-AS1 expression in gastric cancer tissues and cell lines.
  • In vitro assays (migration, invasion, proliferation) to evaluate TONSL-AS1 function.
  • In vivo tumorigenesis assays to confirm tumor-suppressive effects.
  • Analysis of the relationship between TONSL-AS1 and its neighboring gene TONSL.

Main Results:

  • TONSL-AS1 was significantly downregulated in gastric cancer tissues and cell lines.
  • Overexpression of TONSL-AS1 suppressed gastric cancer cell migration, invasion, and proliferation.
  • TONSL-AS1 inhibited tumor formation in vivo.
  • TONSL-AS1 positively regulated the expression of the tumor suppressor gene TONSL.

Conclusions:

  • TONSL-AS1 functions as a tumor suppressor in gastric cancer.
  • The tumor-inhibiting effect of TONSL-AS1 is mediated through the regulation of TONSL.
  • TONSL-AS1 represents a promising novel therapeutic target for gastric cancer.
Keywords:
Gastric cancerLong non-coding RNATONSLTONSL-AS1

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