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Activity and distribution pattern of enzymes in the in-situ pellicle of children

Susann Hertel1, Annika Schulz2, Roman Lang2

  • 1Clinic of Operative Dentistry, Medical Faculty Carl Gustav Carus, TU Dresden, Fetscherstraße 74, 01307 Dresden, Germany.

Archives of Oral Biology
|June 4, 2019
PubMed

Insights

Dental pellicle enzyme activity and structure do not appear linked to caries experience in children. Further research is needed to understand specific glucosyltransferase (GTF) isoform roles in childhood caries susceptibility.

Area of Science:

  • Oral biology
  • Microbiology
  • Biochemistry

Background:

  • Dental caries is a prevalent childhood disease.
  • The acquired pellicle plays a role in oral health.
  • Pellicle enzymes may influence caries development.

Purpose of the Study:

  • To investigate pellicle enzyme activity, distribution, and fluorescence in children with varying caries experience.
  • To examine pellicle ultrastructure and enzyme-bacteria interactions.
  • To establish correlations between pellicle characteristics and caries status.

Main Methods:

  • In-situ pellicle collection from 41 children (4-6 years) with different caries levels.
  • Analysis of amylase, lysozyme, peroxidase, and glucosyltransferase (GTF) activities.
  • Transmission electron microscopy (TEM) and immunofluorescence labeling for enzyme distribution and ultrastructure.

Main Results:

  • No significant differences in overall pellicle enzyme activities were found across caries groups.
  • GTF C and D were absent in the pellicle of caries-active children.
  • Lysozyme exhibited clustered distribution, while other enzymes were randomly distributed; ultrastructure was similar across groups.

Conclusions:

  • Pellicle enzyme activities and ultrastructure are not directly associated with caries status in children.
  • Further research on specific GTF isoforms is recommended to clarify their role in caries susceptibility.
Abstract

Keywords:
BioadhesionChildrenEnzymesFluorescence microscopyPellicleTEM

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