Related Experiment Video
Updated: Jan 24, 2026

Fluorescence Lifetime Macro Imager for Biomedical Applications
Published on: April 7, 2023
Modulation of fluorescence sensing properties of coumarin-based fluorescent probe for H2S and its application in cell
Lei Jin1, Xiaoxue Tan1, Lihui Dai1
1School of Pharmacy, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Yancheng Teachers' University, Yancheng, Jiangsu 224051, People's Republic of China.
Abstract:
A coumarin-based turn on probe, named 3-((E)-3-(1H-indol-3-yl)acryloyl)-2H-chromen-2-one(IAC) was designed and synthesized for the detection of H2S in aqueous medium. IAC showed no obvious red fluorescence in Tris-HCl(pH = 7.2, 60% DMF), a 28.2-fold fluorescence enhancement was found when 40 equiv. H2S was added. Other analytes such as anions, metal ions and GSH, Cys, Gly did no significant fluorescence enhancement at 580 nm to IAC. The red fluorescence enhancement mechanism between IAC and H2S was considered by DFT and ESI-MS. Overall, IAC was successfully applied in cell imaging.
Related Concept Videos
The Sense of Self: Reflected Self-Appraisal and Social Comparison
Photoluminescence: Fluorescence and Phosphorescence
A pair of electrons in a...
Atomic Fluorescence Spectroscopy
Confocal Fluorescence Microscopy
Variables Affecting Phosphorescence and Fluorescence
Fluorescence and Phosphorescence: Instrumentation

