Detection of Arcobacter spp. in Ground Pork by Modified Plating Methods.
C I Collins1, I V Wesley2, E A Murano1
1Iowa State University, Department of Microbiology, Immunology, and Preventive Medicine, Iowa State University, Ames, IA 50011.
Journal of Food Protection
|June 5, 2019
Summary
A new cefsulodin-irgasan-novobiocin (CIN) medium effectively recovers Arcobacter spp. from meats. This study found high Arcobacter prevalence in one pork plant, suggesting targeted interventions are needed.
Area of Science:
- Food Microbiology
- Bacteriology
- Veterinary Public Health
Background:
- Arcobacter spp. are emerging foodborne pathogens.
- Accurate detection methods are crucial for food safety.
- Previous isolation methods for Arcobacter spp. have limitations.
Purpose of the Study:
- To develop and evaluate a modified cefsulodin-irgasan-novobiocin (CIN) medium for Arcobacter spp. recovery from meat.
- To compare the efficacy of the modified CIN medium against other selective and non-selective media.
- To assess the prevalence of Arcobacter spp. in pork processing environments.
Main Methods:
- Development of a modified CIN medium.
- Comparison of modified CIN with brain heart infusion agar supplemented with antibiotics (CVA) and without antibiotics.
- Survey of ground pork samples from multiple pork-processing plants.
Main Results:
- The modified CIN medium demonstrated effectiveness in recovering Arcobacter spp.
- High prevalence (89%) of Arcobacter spp. was detected in ground pork from one specific plant (Plant #1).
- Subsequent surveys showed a significant decrease in prevalence in other plants (5%) but sustained high prevalence (90%) in Plant #1.
Conclusions:
- Modified CIN medium is a valuable tool for Arcobacter spp. detection in meat products.
- Significant variations in Arcobacter spp. prevalence exist between different pork processing facilities.
- Further investigation is needed to identify factors contributing to Arcobacter spp. prevalence, such as farm-level practices or plant-specific sanitation.
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