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Updated: Jan 24, 2026

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Efficient Nondestructive Sampler for Carcasses and Other Surfaces.

A N Sharpe1, C Isigidi Bin Kingombe2, P Watney3

  • 1Bureau of Microbial Hazards, Food Directorate, Health Protection Branch, Health Canada, Tunney's Pasture, Ottawa, Ontario K1A 0L2.

Journal of Food Protection
|June 5, 2019
PubMed
Summary
This summary is machine-generated.

The Rotorinser device effectively removes bacteria from food surfaces like pork and beef, outperforming traditional swabs and stomaching methods for improved microbiological analysis. This electrically powered tool offers superior bacterial removal with minimal tissue damage.

Keywords:
Carcassrotorinserstomachersurface samplingswabtotal counts

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Area of Science:

  • Microbiology
  • Food Safety
  • Instrumentation

Background:

  • Accurate microbiological analysis of food surfaces is crucial for ensuring food safety.
  • Traditional sampling methods like excision and stomaching can be labor-intensive and may not be optimal for in situ analysis.
  • Electrically powered devices offer potential for more efficient and standardized surface sampling.

Purpose of the Study:

  • To evaluate the bacterial removal efficiency of two Rotorinser device versions compared to excision and stomaching.
  • To assess the impact of different sampling protocols, including prewetting and diluents, on bacterial removal.
  • To determine the suitability of the Rotorinser for in situ microbiological sampling of carcasses and other surfaces.

Main Methods:

  • Two versions of the Rotorinser device were tested on pig skin and beef carcass tissue.
  • Bacterial removal efficiency was quantified using a formula comparing Rotorinser counts to stomached excised tissue counts.
  • Various conditions were tested, including prewetting surfaces, enzyme mixtures, and different diluents (NaCl, NaCl-Tween 80) with varying contact times.

Main Results:

  • Both Rotorinser versions demonstrated significantly higher bacterial removal efficiencies than traditional swabs.
  • Rotorinser 1 achieved removal efficiencies of 0.79–0.88 for beef and 0.79–0.95 for pork.
  • Rotorinser 2, using NaCl or NaCl-Tween 80, showed superior performance (0.93–0.98) compared to stomaching (0.86) for removing aerobic mesophilic bacteria from pork skin.
  • Prewetting improved removal, while enzyme mixtures did not.

Conclusions:

  • The Rotorinser is a highly effective tool for removing bacteria from food surfaces, outperforming conventional methods.
  • The device is suitable for in situ sampling, causing minimal tissue damage and adaptable to various surface angles.
  • Optimized protocols, such as using specific diluents and contact times, enhance the Rotorinser's bacterial removal capabilities.