[The protective effect of interleukin-5 in septic mice]

To investigate the protective effect of interleukin-5 (IL-5) in septic mice, male C57BL/6 mice were divided into three groups including sepsis group with intraperitoneal injection of lipopolysaccharide (LPS, 20 mg/kg), study group with combined IL-5 (1 μg per mouse) and LPS (20 mg/kg), control group with normal saline.Pathological changes were tested including lung, heart and kidney tissues. The serum cardiac troponin I (cTnI), serum creatinine (SCr), blood urea nitrogen (BUN), tumor necrosis factor α (TNFα), IL-6, IL-1β, and the chemokine (C-X-C motif) ligand 1(CXCL1) were measured and the survival at 7 d after treatment were recorded. Compared with sepsis group, tissue damages of lung, heart and kidney in IL-5 intervention group were ameliorated. The cTnI, SCr, BUN levels in study group were significantly decreased at 24 h (P<0.05).In addition,TNFα was significantly decreased at 3 h (P<0.05), and CXCL1 decreased at 12 h (P<0.05), while IL-6 and IL-1β were similar at each time point (P>0.05). The mortality at 7 d was less in IL-5 treatment group. In summary, IL-5 has a protective effect and reduces the inflammatory response in septic mice.