Effect of mixing duration on biogas production and methanogen distribution in an anaerobic digester

Mixing has been shown to have effect on biogas production in anaerobic digestion systems. To further examine this impact, a study was designed to evaluate nearly continuous mixing (mixing for 15 min followed by no mixing for 15 min, CON), intermediate mixing (mixing for 15 min followed by no mixing for 45 min, INT) and no mixing (unmixed, NO) on biogas production in three 208 L pilot-scale tank reactors. The experiments were conducted in triplicates at a controlled temperature of 37 ± 1°C, with a total solids percentage of 5%, a hydraulic retention time (HRT) of 21 days, and an organic loading rate (OLR) of 2 kg VS m^-3 d^{- 1}. Digesters with NO mixing had greater solids build up in the bottom quarter of the digester after four weeks' retention time. The methane percentage in biogas produced from digesters with INT and CON mixing were 63% and 62%, respectively, which were 4% and 5% higher than that from digesters with NO mixing (58%). The specific methane yield for digesters with NO, INT and CON mixing was 1.15, 1.15, and 1.49 m³-methane per kg-VS destroyed, however, those differences were not statistically significant (p > 0.05). Digesters had the least amount of Methanosarcinales of the methanogens measured under all treatments. However, the Methanosarcinales, Methanosarcinaceae, Methanomicrobiales, and the total amount of methanogens were less in digesters with INT mixing compared to NO and CON mixing treatments.