Related Experiment Video
Updated: Jan 23, 2026

Author Spotlight: Microbial Control and Monitoring Strategies for Cleanroom Environments and Cellular Therapies
Published on: March 17, 2023
Principles of and strategies for germline gene therapy
Don P Wolf1, Paul A Mitalipov1, Shoukhrat M Mitalipov2
1Center for Embryonic Cell and Gene Therapy, Oregon Health & Science University, Portland, OR, USA.
Abstract:
Monogenic disorders occur at a high frequency in human populations and are commonly inherited through the germline. Unfortunately, once the mutation has been transmitted to a child, only limited treatment options are available in most cases. However, means of correcting disease-causing nuclear and mitochondrial DNA mutations in gametes or preimplantation embryos have now been developed and are commonly referred to as germline gene therapy (GGT). We will discuss these novel strategies and provide a path forward for safe, high-efficiency GGT that may provide a promising new paradigm for preventing the passage of deleterious genes from parent to child.
Related Concept Videos
Gene Therapy
Hardy-Weinberg Principle
The Uncertainty Principle
The Pauli Exclusion Principle
The Aufbau Principle and Hund's Rule
Le Chatelier's Principle: Changing Concentration

