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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
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Phylogenetic Trees03:21

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Effects of EDTA on End-Point Detection Methods01:18

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Different methods, such as visual observance of metal-ion indicators, spectroscopic techniques, and potentiometric methods, can determine the endpoint of an EDTA titration.
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Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
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Buffer solutions do not have an unlimited capacity to keep the pH relatively constant . Instead, the ability of a buffer solution to resist changes in pH relies on the presence of appreciable amounts of its conjugate weak acid-base pair. When enough strong acid or base is added to substantially lower the concentration of either member of the buffer pair, the buffering action within the solution is compromised.
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Framing Effects

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Information is everywhere and its presentation—such as how and when items are presented—can impact our perceptions and decisions surrounding the info. This broad concept umbrellas framing effects—influences that occur due to the way information is framed in its appearance, whether it’s purely the order or the specific wording of a message. Let’s take a look at numerous ways in which two versions of something can objectively say the same thing, yet we respond in...
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Related Experiment Video

Updated: Jan 23, 2026

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture 4C-seq
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Sequence capture using AFLP-generated baits: A cost-effective method for high-throughput phylogenetic and

Jia-Xuan Li1, Zhao-Chi Zeng1, Ying-Yong Wang1

  • 1State Key Laboratory of Biocontrol, College of Ecology and Evolution, School of Life Sciences Sun Yat-Sen University Guangzhou China.

Ecology and Evolution
|June 5, 2019
PubMed
Summary

A new method called AFLP Capture uses Amplified Fragment Length Polymorphism (AFLP) to create custom DNA capture baits, significantly reducing costs for genomic studies. This technique enables efficient phylogenetic analysis across various evolutionary scales.

Keywords:
Odorranabaitphylogeneticspopulation geneticssequence capture

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Generation of Native Chromatin Immunoprecipitation Sequencing Libraries for Nucleosome Density Analysis
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Area of Science:

  • Genomics
  • Evolutionary Biology
  • Molecular Ecology

Background:

  • Target sequence capture enriches genomic regions for high-throughput sequencing.
  • Existing methods often rely on expensive commercial synthetic baits.
  • A cost-effective alternative is needed for broad applicability in ecological and evolutionary studies.

Purpose of the Study:

  • To develop a novel, cost-effective sequence capture method using Amplified Fragment Length Polymorphism (AFLP).
  • To demonstrate the utility of AFLP-based genome sequence capture (AFLP Capture) for phylogenetic studies.
  • To provide a flexible tool applicable to any organism without requiring prior genome information.

Main Methods:

  • Utilized Amplified Fragment Length Polymorphism (AFLP) technique to generate homemade capture baits.
  • Employed biotinylated AFLP fragments to capture homologous sequences from genomic shotgun libraries.
  • Applied the method to 11 *Odorrana* species for orthologous loci identification and SNP discovery.

Main Results:

  • Successfully obtained 511 orthologous loci across 11 *Odorrana* species.
  • Identified over 100,000 single nucleotide polymorphisms (SNPs) within four individuals of one species.
  • Demonstrated applicability for evolutionary questions at both interspecies and intraspecies levels.

Conclusions:

  • AFLP Capture is a rapid, flexible, and cost-effective tool for phylogenetic studies.
  • Significantly reduces experimental costs for analyzing genome-scale data.
  • Enables high-throughput sequencing applications in diverse organisms without prior genomic data.