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Knockdown of differentiation antagonizing non-protein coding RNA exerts anti-tumor effect by up-regulating miR-214 in

Jingli Sun1, Shaofeng Gao1, Cuihua Lu2

  • 1Department of Obstetrics and Gynecology, Shanxian Central Hospital, No. 1 Wenhua Road, Heze, 274300, Shandong, China.

Molecular and Cellular Biochemistry
|June 5, 2019
PubMed

Insights

Differentiation antagonizing non-protein coding RNA (DANCR) promotes endometrial carcinoma (EC) tumorigenesis by sponging miR-214. Targeting DANCR may offer a new therapeutic strategy for EC treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long noncoding RNAs (lncRNAs) play critical roles in cancer progression.
  • Differentiation antagonizing non-protein coding RNA (DANCR) is implicated in various cancers.
  • The specific role of DANCR in endometrial carcinoma (EC) remains largely unelucidated.

Purpose of the Study:

  • To investigate the function of DANCR in endometrial carcinoma (EC).
  • To explore the underlying molecular mechanism of DANCR in EC tumorigenesis.
  • To assess DANCR as a potential therapeutic target for EC.

Main Methods:

  • Quantitative reverse transcription PCR (qRT-PCR) to determine DANCR expression.
  • Small interfering RNA (siRNA) to knockdown DANCR in EC cell lines (AN3CA, HEC-1B).
  • Cell Counting Kit-8 (CCK-8) assay for proliferation, flow cytometry for apoptosis, and luciferase reporter assay for miRNA interaction.

Main Results:

  • DANCR expression was significantly upregulated in EC tissues compared to normal controls.
  • Knockdown of DANCR suppressed EC cell proliferation and induced apoptosis.
  • DANCR was identified as a sponge for miR-214, which was downregulated in EC; miR-214 suppression reversed the effects of DANCR knockdown.

Conclusions:

  • DANCR promotes EC tumorigenesis by sponging miR-214.
  • DANCR acts as an oncogenic lncRNA in endometrial carcinoma.
  • DANCR represents a potential therapeutic target for EC treatment.
Keywords:
DANCREndometrial carcinomaceRNAlncRNAmiR-214

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