Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Morphogenesis02:19

Morphogenesis

30.3K
Plant morphogenesis—the development of a plant’s form and structure—involves several overlapping developmental processes, including growth and cell differentiation. Precursor cells differentiate into specific cell types, which are organized into the tissues and organ systems that make up the functional plant.
30.3K
Autophagy01:27

Autophagy

5.7K
Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
5.7K
Requirements for Human Life01:26

Requirements for Human Life

13.2K
The Earth and its atmosphere have provided humans with air, water, and food, but these are not the only requirements for survival. Humans also require a specific range of temperature and pressure that the Earth and its atmosphere provides.
Oxygen
Atmospheric air is only about 20 percent oxygen, but that oxygen is a key component of the chemical reactions that keep the body alive, including the reactions that produce ATP. Brain cells are susceptible to a lack of oxygen because they require a...
13.2K
Proteins: Dietary Sources and Requirements01:28

Proteins: Dietary Sources and Requirements

1.6K
Consuming animal-based products offers high-quality proteins that contain optimal levels and combinations of essential amino acids, crucial for tissue repair and growth. Foods like eggs, milk, fish, and most meats are a source of complete proteins. Legumes and cereals are abundant in proteins; however, they typically lack a full range of essential amino acids. As a result, they are considered incomplete protein sources. Some plant sources like soybeans, quinoa, and amaranth do contain complete...
1.6K
Oxygen Requirements and Growth Patterns01:29

Oxygen Requirements and Growth Patterns

1.2K
Microorganisms exhibit diverse oxygen requirements and growth patterns driven by their metabolic strategies and environmental adaptations. Oxygen, while essential for many organisms, can also be toxic under certain conditions, shaping how microorganisms grow and survive.Oxygen Requirements of MicroorganismsMicroorganisms are classified based on their ability to use or tolerate oxygen:● Obligate aerobes like Mycobacterium tuberculosis need oxygen for energy production, as it serves as the...
1.2K
Lung Capacity01:47

Lung Capacity

56.2K
The air in the lungs is measured in volumes and capacities. Lung volume measures reflect the amount of air taken in, released, or left over after a lung function, like a single inhalation. Lung capacity measures are sums of two or more lung volume measures.
56.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inhibition of thrombospondin-1 signaling prevents experimental bronchopulmonary dysplasia.

American journal of physiology. Lung cellular and molecular physiology·2026
Same author

Higher body mass index is associated with lower drug concentrations in patients with inflammatory bowel disease treated with ustekinumab but not vedolizumab.

Inflammatory bowel diseases·2026
Same author

Postpartum nonsteroidal anti-inflammatory drug exposure does not increase risk for flare in patients with inflammatory bowel disease.

Annals of gastroenterology·2026
Same author

Explant Culture for Studying Lung Development.

Methods in molecular biology (Clifton, N.J.)·2026
Same author

Correction: Early detection of hypovolemia by venous pulse wave velocity and vena cava ultrasound imaging.

European journal of applied physiology·2026
Same author

A Bloom Beneath the Skin.

Clinical infectious diseases : an official publication of the Infectious Diseases Society of America·2026

Related Experiment Video

Updated: Jan 23, 2026

Activating Autophagy by Aerobic Exercise in Mice
08:44

Activating Autophagy by Aerobic Exercise in Mice

Published on: February 3, 2017

12.7K

Autophagy is required for lung development and morphogenesis.

Behzad Yeganeh1, Joyce Lee1,2, Leonardo Ermini1

  • 1Program in Translational Medicine, Peter Gilgan Centre for Research and Learning, Hospital for Sick Children.

The Journal of Clinical Investigation
|June 5, 2019
PubMed
Summary
This summary is machine-generated.

Autophagy, a cellular process, is crucial for normal lung development in mice. Impaired autophagy is linked to respiratory problems similar to bronchopulmonary dysplasia (BPD) in premature infants.

Keywords:
AutophagyDevelopmentOrganogenesisPulmonary surfactantsPulmonology

More Related Videos

Use of Shigella flexneri to Study Autophagy-Cytoskeleton Interactions
12:52

Use of Shigella flexneri to Study Autophagy-Cytoskeleton Interactions

Published on: September 9, 2014

16.4K
Author Spotlight: Optimizing Bovine Lung Decellularization for Organotypic Hydrogels
06:12

Author Spotlight: Optimizing Bovine Lung Decellularization for Organotypic Hydrogels

Published on: December 8, 2023

2.2K

Related Experiment Videos

Last Updated: Jan 23, 2026

Activating Autophagy by Aerobic Exercise in Mice
08:44

Activating Autophagy by Aerobic Exercise in Mice

Published on: February 3, 2017

12.7K
Use of Shigella flexneri to Study Autophagy-Cytoskeleton Interactions
12:52

Use of Shigella flexneri to Study Autophagy-Cytoskeleton Interactions

Published on: September 9, 2014

16.4K
Author Spotlight: Optimizing Bovine Lung Decellularization for Organotypic Hydrogels
06:12

Author Spotlight: Optimizing Bovine Lung Decellularization for Organotypic Hydrogels

Published on: December 8, 2023

2.2K

Area of Science:

  • Developmental Biology
  • Cellular Biology
  • Neonatal Respiratory Medicine

Background:

  • Bronchopulmonary dysplasia (BPD) is a significant respiratory morbidity in extremely premature infants.
  • The precise biological mechanisms underlying BPD, particularly concerning lung development, are not fully elucidated.
  • An arrest in lung development is hypothesized to contribute to the pathogenesis of BPD.

Purpose of the Study:

  • To investigate the role and timing of autophagy during mouse lung development.
  • To determine the regulatory function of autophagy in airway branching and terminal sacculi formation.
  • To explore the potential link between impaired autophagy and the development of BPD-like pathology.

Main Methods:

  • Examined epithelial autophagy activation in developing mouse lungs, correlating it with AMPK activation.
  • Utilized in vitro models to assess the impact of inhibiting AMPK-mediated autophagy on lung branching.
  • Generated conditional knockout mouse models (Becn1Epi-KO) at different gestational ages (E10.5 and E16.5) to study the effects of beclin 1 deletion in lung epithelial cells.

Main Results:

  • Identified two critical windows of epithelial autophagy activation, both dependent on AMPK, during mouse lung development.
  • Inhibition of autophagy impaired in vitro lung branching; genetic deletion of beclin 1 (Becn1) led to lethal respiratory distress.
  • Early Becn1 deletion (E10.5) resulted in defective airway branching, sacculi formation, vascularization, increased cell death, and delayed maturation; late deletion (E16.5) caused impaired air sac formation and delayed differentiation, mimicking BPD pathology.

Conclusions:

  • Intrinsic autophagy is a vital regulator of lung development and morphogenesis.
  • Impairment of autophagy during critical developmental stages contributes to pathologies resembling bronchopulmonary dysplasia.
  • These findings highlight autophagy as a potential therapeutic target for preventing or treating BPD.