Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Plasmid replication stimulates DNA recombination in Bacillus subtilis.

P Noirot1, M A Petit, S D Ehrlich

  • 1Institut Jacques Monod, Paris, France.

Journal of Molecular Biology
|July 5, 1987
PubMed
Summary

Plasmid replication significantly boosts homologous recombination in Bacillus subtilis. This study demonstrates that active plasmid replication, when near DNA repeats, increases recombination frequency by 20 to 450 times.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Efficient transformation of Lactobacillus sake by electroporation.

Microbiology (Reading, England)·2021
Same author

Correction: New insights into intestinal phages.

Mucosal immunology·2020
Same author

New insights into intestinal phages.

Mucosal immunology·2020
Same author

Specific gut microbiota features and metabolic markers in postmenopausal women with obesity.

Nutrition & diabetes·2015
Same author

The association of serum 25-hydroxyvitamin D with indicators of bone quality in men of Caucasian and African ancestry.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2010
Same author

Biological underpinnings of Frost's mechanostat thresholds: the important role of osteocytes.

Journal of musculoskeletal & neuronal interactions·2010

Area of Science:

  • Molecular Biology
  • Genetics
  • Microbiology

Background:

  • Homologous recombination is a fundamental DNA repair mechanism.
  • The influence of extrachromosomal elements like plasmids on recombination is not fully understood.
  • Understanding these interactions is crucial for genetic engineering and understanding genome stability.

Purpose of the Study:

  • To investigate the impact of plasmid replication on homologous recombination frequency.
  • To determine if plasmid proximity to DNA repeats affects recombination rates.
  • To elucidate the role of plasmid replication activity in stimulating genetic recombination.

Main Methods:

  • Constructed Bacillus subtilis strains with chromosomal direct DNA repeats.
  • Integrated a thermosensitive plasmid (pE194) near or distant to the repeats.

Related Experiment Videos

  • Regulated plasmid replication by controlling temperature.
  • Quantified recombination frequency between direct repeats under different replication conditions.

    • Main Results:

    • Recombination frequency was low (approx. 10^-4 per generation) when plasmid pE194 replication was inactive.
    • Recombination frequency increased 20 to 450-fold when plasmid pE194 replication was active.
    • The stimulatory effect was observed when the plasmid was located in proximity to the DNA repeats.

    Conclusions:

    • Plasmid replication actively stimulates homologous recombination.
    • The proximity of the replicating plasmid to homologous sequences enhances this stimulation.
    • These findings highlight a novel mechanism by which plasmids can influence genomic rearrangements.