Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

2D NMR: Homonuclear Correlation Spectroscopy (COSY)01:06

2D NMR: Homonuclear Correlation Spectroscopy (COSY)

1.9K
Homonuclear correlation spectroscopy, or COSY, is a 2-dimensional NMR technique that provides information about coupled protons. Typically, the geminal and vicinal coupling are observed. For example, consider the COSY spectrum of ethyl acetate, where its 1D proton NMR spectrum is plotted along the vertical and horizontal axes with their corresponding chemical shift scale. Three spots on the diagonal corresponding to the three peaks in the 1D proton spectrum are called diagonal peaks. The COSY...
1.9K
Atomic Fluorescence Spectroscopy01:29

Atomic Fluorescence Spectroscopy

933
Atomic fluorescence spectroscopy (AFS) is an analytical technique that involves the electronic transitions of atoms in a flame, furnace, or plasma being excited by electromagnetic (EM) radiation. When these atoms absorb energy, they become excited and subsequently release energy as they return to their original state. This emitted light, or "fluorescence," is observed at a right angle to the incident beam. Both absorption and emission processes transpire at distinct wavelengths, which...
933
Correlations02:20

Correlations

35.8K
Correlation means that there is a relationship between two or more variables (such as ice cream consumption and crime), but this relationship does not necessarily imply cause and effect. When two variables are correlated, it simply means that as one variable changes, so does the other. We can measure correlation by calculating a statistic known as a correlation coefficient. A correlation coefficient is a number from -1 to +1 that indicates the strength and direction of the relationship between...
35.8K
2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)01:19

2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

1.4K
Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...
1.4K
Correlation and Causation01:27

Correlation and Causation

42.3K
Statistical tests can calculate whether there is a relationship, or correlation, between independent and dependent variables. An indirect relationship of the variables signifies a correlation, while a direct relationship shows causation. If it is determined that no connection exists between the variables, then the correlation is a coincidence.
Correlation versus Causation
If the dependent variable increases or decreases when the independent variable increases, there is a positive or negative...
42.3K
Atomic Spectroscopy: Absorption, Emission, and Fluorescence01:23

Atomic Spectroscopy: Absorption, Emission, and Fluorescence

2.7K
Atomic spectroscopy is a vital tool in elemental analysis, both qualitatively and quantitatively. It can be broadly divided into optical spectroscopy, mass spectroscopy, and X-ray spectroscopy methods. The optical spectroscopic methods are atomic absorption spectroscopy (AAS), atomic emission spectroscopy (AES), and atomic fluorescence spectroscopy (AFS). The first step in all three methods is atomization, where the solid, liquid, or solution-phase samples are converted into gas-phase atoms and...
2.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fluorescent protein-based probe architecture modulates rotational and translational diffusion readouts in polarization-dependent fluorescence correlation spectroscopy.

Microscopy (Oxford, England)·2026
Same author

Publisher's Note: "Non-thermal acceleration of DNA base pairing by sub-terahertz irradiation" [J. Chem. Phys. 164, 065102 (2026)].

The Journal of chemical physics·2026
Same author

Title: Non-thermal acceleration of DNA base pairing by sub-terahertz irradiation.

The Journal of chemical physics·2026
Same author

Microtubule Deformation Modulates Intracellular Transport by Kinesin Differently Than Dynein.

IEEE transactions on nanobioscience·2025
Same author

Short Repeat Ribonucleic Acid Reduces Cytotoxicity by Preventing the Aggregation of TDP-43 and Its 25 KDa Carboxy-Terminal Fragment.

JACS Au·2024
Same author

Hetero-oligomerization of TDP-43 carboxy-terminal fragments with cellular proteins contributes to proteotoxicity.

Communications biology·2024

Related Experiment Video

Updated: Jan 23, 2026

Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy
14:04

Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy

Published on: April 25, 2021

6.1K

Full fiber-optic fluorescence correlation spectroscopy.

Johtaro Yamamoto, Masataka Kinjo

    Optics Express
    |June 6, 2019
    PubMed
    Summary
    This summary is machine-generated.

    A new full fiber-optic fluorescence correlation spectroscopy (FF-FCS) technique simplifies measurements without objectives. This compact FF-FCS system is feasible for laboratory and quality-control applications.

    More Related Videos

    Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
    10:21

    Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

    Published on: May 5, 2016

    11.2K
    Cerebral Blood Flow-Based Resting State Functional Connectivity of the Human Brain using Optical Diffuse Correlation Spectroscopy
    07:13

    Cerebral Blood Flow-Based Resting State Functional Connectivity of the Human Brain using Optical Diffuse Correlation Spectroscopy

    Published on: May 27, 2020

    7.1K

    Related Experiment Videos

    Last Updated: Jan 23, 2026

    Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy
    14:04

    Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy

    Published on: April 25, 2021

    6.1K
    Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
    10:21

    Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

    Published on: May 5, 2016

    11.2K
    Cerebral Blood Flow-Based Resting State Functional Connectivity of the Human Brain using Optical Diffuse Correlation Spectroscopy
    07:13

    Cerebral Blood Flow-Based Resting State Functional Connectivity of the Human Brain using Optical Diffuse Correlation Spectroscopy

    Published on: May 27, 2020

    7.1K

    Area of Science:

    • Optical Spectroscopy
    • Biophotonics
    • Analytical Chemistry

    Background:

    • Conventional fluorescence correlation spectroscopy (FCS) relies on complex optical setups involving objectives and dichroic mirrors.
    • There is a need for simplified, miniaturized spectroscopic tools for broader accessibility and integration into various systems.

    Purpose of the Study:

    • To develop and demonstrate a simplified full fiber-optic fluorescence correlation spectroscopy (FF-FCS) technique.
    • To eliminate the need for traditional objectives and dichroic mirrors in FCS measurements.
    • To assess the feasibility of FF-FCS for practical applications.

    Main Methods:

    • Developed a novel FF-FCS system utilizing a lensed optical fiber or gradient index (GRIN) lens for laser focusing.
    • Eliminated the use of objectives and dichroic mirrors in the optical path.
    • Validated the FF-FCS system by measuring fluorescent beads.

    Main Results:

    • Successfully demonstrated the feasibility of the FF-FCS technique.
    • The FF-FCS system is significantly simpler and smaller compared to conventional FCS.
    • Achieved comparable performance to conventional FCS, though not higher sensitivity.

    Conclusions:

    • The developed FF-FCS technique offers a simplified and miniaturized approach to fluorescence correlation spectroscopy.
    • FF-FCS is a viable alternative for applications where simplicity and size are critical.
    • Potential for widespread adoption as a laboratory tool and embedded system for quality control, such as in cytometers.