Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Characteristics of Practical Op Amps

Characteristics of Practical Op Amps

A difference amplifier, a crucial component in numerous electronic devices, ideally amplifies only the difference-mode signal, which is the difference between two input signals. However, in practical circuits, the output voltage depends on both the differential gain and the common-mode gain.
The ratio of differential gain to the common-mode gain is defined as the common-mode rejection ratio (CMRR). This ratio quantifies the ability of operational amplifiers (op-amps) to reject common-mode...

Theoretical Foundations of Nursing Practice

Theoretical Foundations of Nursing Practice

Theories play an essential role in organizing patient care. Theories refer to a proposed or followed belief, policy, or procedure that is the basis for action. Nursing theories are knowledge-based concepts that guide nurses' actions, influence nursing education and practice, and allow nurses to care for their patients.
Theories provide a perspective to assess patients' conditions and organize data and methods. They also assist in analyzing and interpreting information. They represent a...

Equivalent Circuits for Practical Transformers

Equivalent Circuits for Practical Transformers

The practical equivalent circuits of single-phase two-winding transformers exhibit significant deviations from their idealized versions due to the inherent properties of winding resistance and finite core permeability. These properties result in real and reactive power losses, affecting the transformer's performance. Understanding these deviations is crucial for designing more efficient transformers.
In a practical transformer, each winding exhibits resistance and leakage reactance. The...

Methods of Sterilization I: Physical Methods

Methods of Sterilization I: Physical Methods

As used in a healthcare facility, sterilization destroys all microorganisms through physical or chemical methods. The physical method includes steam, dry heat, boiling water, and radiation.
Steam sterilization uses non-toxic, low-cost moist heat in the form of saturated steam under pressure, which is fast, microbicidal, and sporicidal, and quickly warms and penetrates fabrics. Autoclaves, or steam sterilizers, expose each item to direct steam contact for a predetermined time at the necessary...

The Scientific Method

The Scientific Method

Chemistry is an empirical science. Scientists often pose questions to understand the chemistry in everyday life and seek answers to these questions. To achieve this, scientists follow a definitive series of steps that together make up the Scientific Method. This approach involves making observations, asking questions, building a hypothesis, conducting experiments, analyzing results, and forming a conclusion.

The Scientific Method

The Scientific Method

The scientific method is a detailed, empirical problem-solving process used by biologists and other scientists. This iterative approach involves formulating a question based on observation, developing a testable potential explanation for the observation (called a hypothesis), making and testing predictions based on the hypothesis, and using the findings to create new hypotheses and predictions.
Generally, predictions are tested using carefully-designed experiments. Based on the outcome of these...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Plasmodium-induced disruption of brain endothelial barrier integrity in vitro and in mice is prevented by inhibitors of farnesyltransferase.

Malaria journal·2026

Same author

Co(II), Cu(II), and Ni(II) Coordination Complexes: Synthesis, Characterization, Experimental, and Computational Study on Potential Antiplasmodial Activity.

ChemMedChem·2026

Same author

Synthesis, Characterization, and Anti-Plasmodium falciparum Activity of a New Copper(II) Complex Containing 2-(Tert-Butoxy)-6-(1H-imidazol-1-yl)pyridine.

ChemMedChem·2026

Same author

Bacteriocin presence enhances phage-related risks in dairy fermentations.

Food research international (Ottawa, Ont.)·2026

Same author

<i>Plasmodium falciparum</i> hemozoin-associated biomolecules induce brain endothelial cell barrier disruption in an <i>in vitro</i> model of cerebral malaria.

mBio·2026

Same author

Interprofessional Workshop: An Impactful Approach to Introducing Interprofessional Education to Health Professions Students.

Southern medical journal·2026

Search research articles

Related Experiment Video

Updated: Jan 23, 2026

Author Spotlight: Advancing Protein Engineering – Harnessing Evolution Through PRANCE and Lab Automation

Author Spotlight: Advancing Protein Engineering – Harnessing Evolution Through PRANCE and Lab Automation

Published on: January 12, 2024

Practical Method for Isolation of Phage Deletion Mutants.

Diana Gutiérrez¹, Lucía Fernández², Ana Rodríguez³

¹Instituto de Productos Lácteos de Asturias (IPLA-CSIC), Paseo Río Linares s/n, 33300 Villaviciosa, Spain. dianagufer@ipla.csic.es.

Methods and Protocols

|June 6, 2019

Summary

This study introduces a method to create bacteriophage (phage) mutants with deletions, aiding in understanding phage biology and developing phage therapy against multi-drug resistant bacteria.

Keywords:

chelating agent phage deletion mutant temperate phage virulent phage

More Related Videos

Characterize Disease-related Mutants of RAF Family Kinases by Using a Set of Practical and Feasible Methods

Characterize Disease-related Mutants of RAF Family Kinases by Using a Set of Practical and Feasible Methods

Published on: July 17, 2019

Rapid Deletion Production in Fungi via Agrobacterium Mediated Transformation of OSCAR Deletion Constructs

Rapid Deletion Production in Fungi via Agrobacterium Mediated Transformation of OSCAR Deletion Constructs

Published on: June 12, 2017

Related Experiment Videos

Last Updated: Jan 23, 2026

Author Spotlight: Advancing Protein Engineering – Harnessing Evolution Through PRANCE and Lab Automation

Author Spotlight: Advancing Protein Engineering – Harnessing Evolution Through PRANCE and Lab Automation

Published on: January 12, 2024

Characterize Disease-related Mutants of RAF Family Kinases by Using a Set of Practical and Feasible Methods

Characterize Disease-related Mutants of RAF Family Kinases by Using a Set of Practical and Feasible Methods

Published on: July 17, 2019

Rapid Deletion Production in Fungi via Agrobacterium Mediated Transformation of OSCAR Deletion Constructs

Rapid Deletion Production in Fungi via Agrobacterium Mediated Transformation of OSCAR Deletion Constructs

Published on: June 12, 2017

Area of Science:

Microbiology
Genetics
Biotechnology

Background:

Multi-drug resistant bacteria pose a significant threat, driving interest in bacteriophages (phages) for antimicrobial applications and phage therapy.
In-depth genomic characterization of phages is crucial for their therapeutic use, requiring functional gene assignment and screening for undesirable genes.

Purpose of the Study:

To develop a simple protocol for selecting phage mutants with random genomic deletions.
To facilitate the functional analysis of phage genes and ensure the absence of virulence or antibiotic resistance genes.
To enable the selection of lytic variants from temperate phages for enhanced antimicrobial efficacy.

Main Methods:

A protocol utilizing the instability of phage particles in the presence of chelating compounds to induce random DNA deletions.
Selection of phage mutants that retain particle viability after treatment, indicating successful deletion of non-essential genomic regions.

Main Results:

Demonstrated a method to generate phage mutants with random deletions across their genomes.
Successfully applied the protocol to obtain mutations in genes involved in the lysogenic cycle of phages infecting *Staphylococcus* and *Lactobacillus*.

Conclusions:

The described protocol provides a straightforward approach for genomic manipulation of bacteriophages.
This technique is valuable for unraveling phage biology, ensuring phage safety, and optimizing phages for therapeutic applications, particularly for combating antibiotic resistance.