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Updated: Jan 23, 2026

Silencing the Spark: CRISPR/Cas9 Genome Editing in Weakly Electric Fish
Published on: October 27, 2019
CRISPR-Cas9 Mediated Genome Editing in Bicyclus anynana Butterflies
Tirtha Das Banerjee1, Antónia Monteiro2,3
1Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543, Singapore. tirtha_banerjee@u.nus.edu.
Abstract:
CRISPR-Cas9 is revolutionizing the field of genome editing in non-model organisms. The robustness, ease of use, replicability and affordability of the technology has resulted in its widespread adoption among researchers. The African butterfly Bicyclus anynana is an emerging model lepidopteran species in the field of evo-devo, with a sequenced genome and amenable to germ line transformation. However, efficient genome editing tools to accelerate the pace of functional genetic research in this species have only recently become available with CRISPR-Cas9 technology. Here, we provide a detailed explanation of the CRISPR-Cas9 protocol we follow in the lab. The technique has been successfully implemented to knock-out genes associated with eyespot development and melanin pigmentation.
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