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Characterization of the small RNA of the bacteriophage phi 29 DNA packaging machine
P X Guo1, S Bailey, J W Bodley
1Department of Microbiology/Dentistry, University of Minnesota, Minneapolis 55455.
Nucleic Acids Research
|September 11, 1987
Abstract:
The prohead connector of the bacteriophage luminal diameter 29 DNA packaging machine was reconstructed with the small RNA that regulates DNA packaging in vitro. The complete sequence of the 120 nucleotide RNA proved its origination from the promoter PE1(A1) of the left early region of phi 29 DNA, the end packaged first during assembly. The prohead RNA was clearly distinct from eubacterial 5S rRNA in sequence and composition.