Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Piaget's Stage 1 of Cognitive Development01:14

Piaget's Stage 1 of Cognitive Development

1.7K
The sensorimotor stage, the initial phase of Jean Piaget's theory of cognitive development, spans the first two years of a child's life. During this period, infants actively engage with their surroundings, building cognitive awareness through direct interaction with the world. This interaction is primarily based on sensory perception and motor actions, allowing infants to gradually understand basic physical properties and predict how objects interact within their environment.
Exploration...
1.7K
The Eukaryotic Promoter Region02:40

The Eukaryotic Promoter Region

18.7K
The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences.  The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
18.7K
The Eukaryotic Promoter Region02:40

The Eukaryotic Promoter Region

3.8K
3.8K
Lineage Commitment01:21

Lineage Commitment

4.2K
Commitment is the  process whereby stem cells:
4.2K
The Tumor Microenvironment02:17

The Tumor Microenvironment

7.7K
Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
7.7K
Frequency-dependent Selection01:21

Frequency-dependent Selection

23.2K
When the fitness of a trait is influenced by how common it is (i.e., its frequency) relative to different traits within a population, this is referred to as frequency-dependent selection. Frequency-dependent selection may occur between species or within a single species. This type of selection can either be positive—with more common phenotypes having higher fitness—or negative, with rarer phenotypes conferring increased fitness.
23.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Potential role of palmitoylation-coexpressed lncRNA in prognosis and immunotherapy of hepatocellular carcinoma.

Computer methods in biomechanics and biomedical engineering·2026
Same author

Depletion of macrophages during early postnatal development leads to disrupted tooth root development and altered Gli1⁺ MSC trajectory.

Cell death & disease·2026
Same author

Correction: Loss of p53 in mesenchymal stem cells promotes alteration of bone remodeling through negative regulation of osteoprotegerin.

Cell death and differentiation·2026
Same author

Dexamethasone promotes neutrophil ROS-mediated tumor killing through the glucocorticoid receptor.

Oncogene·2026
Same author

Neuropilin-2 Deficiency Promotes Mitochondrial Dysfunction and NAD⁺-Dependent Cellular Senescence in Retinal Degeneration.

Investigative ophthalmology & visual science·2026
Same author

Trump, Coca-Cola and the fructose frenzy that influences brain and neuronal tumor development.

Cell death and differentiation·2026

Related Experiment Video

Updated: Jan 23, 2026

Development of an Ethanol-induced Fibrotic Liver Model in Zebrafish to Study Progenitor Cell-mediated Hepatocyte Regeneration
10:42

Development of an Ethanol-induced Fibrotic Liver Model in Zebrafish to Study Progenitor Cell-mediated Hepatocyte Regeneration

Published on: May 13, 2016

9.5K

Fibrotic liver microenvironment promotes Dll4 and SDF-1-dependent T-cell lineage development.

Zheng Gong1,2, Bingxue Shang1, Yunpeng Chu1

  • 1The First Affiliated Hospital of Soochow University, Institutes for Translational Medicine, State Key Laboratory of Radiation Medicine and Protection, Key Laboratory of Stem Cells and Medical Biomaterials of Jiangsu Province, Soochow University Medical College, Suzhou, China.

Cell Death & Disease
|June 6, 2019
PubMed
Summary

Restoring T-cells is crucial for HIV, cancer, and aging. Bone marrow transfusion via the hepatic portal vein enhances T-cell reconstitution, particularly in fibrotic livers, by upregulating Dll4 and SDF-1.

More Related Videos

Analysis of Liver Microenvironment During Early Progression of Non-Alcoholic Fatty Liver Disease-Associated Hepatocellular Carcinoma in Zebrafish
09:27

Analysis of Liver Microenvironment During Early Progression of Non-Alcoholic Fatty Liver Disease-Associated Hepatocellular Carcinoma in Zebrafish

Published on: April 1, 2021

4.0K
Cell-Lineage Guided Mass Spectrometry Proteomics in the Developing Frog Embryo
09:18

Cell-Lineage Guided Mass Spectrometry Proteomics in the Developing Frog Embryo

Published on: April 21, 2022

2.2K

Related Experiment Videos

Last Updated: Jan 23, 2026

Development of an Ethanol-induced Fibrotic Liver Model in Zebrafish to Study Progenitor Cell-mediated Hepatocyte Regeneration
10:42

Development of an Ethanol-induced Fibrotic Liver Model in Zebrafish to Study Progenitor Cell-mediated Hepatocyte Regeneration

Published on: May 13, 2016

9.5K
Analysis of Liver Microenvironment During Early Progression of Non-Alcoholic Fatty Liver Disease-Associated Hepatocellular Carcinoma in Zebrafish
09:27

Analysis of Liver Microenvironment During Early Progression of Non-Alcoholic Fatty Liver Disease-Associated Hepatocellular Carcinoma in Zebrafish

Published on: April 1, 2021

4.0K
Cell-Lineage Guided Mass Spectrometry Proteomics in the Developing Frog Embryo
09:18

Cell-Lineage Guided Mass Spectrometry Proteomics in the Developing Frog Embryo

Published on: April 21, 2022

2.2K

Area of Science:

  • Immunology
  • Hepatology
  • Stem Cell Biology

Background:

  • T-cell reconstitution is vital for managing conditions like HIV/AIDS, cancer, and aging.
  • Autologous bone marrow transfusion (BMT) via the hepatic portal vein previously restored CD4+ T-cell counts in AIDS patients with liver cirrhosis.
  • Liver fibrosis presents challenges for effective T-cell restoration.

Purpose of the Study:

  • To investigate T-cell reconstitution in a mouse model of liver fibrosis.
  • To elucidate the role of the fibrotic liver microenvironment in T-cell development.
  • To identify molecular mechanisms promoting T-cell generation in pathological conditions.

Main Methods:

  • Induction of liver fibrosis in mice using carbon tetrachloride (CCl4).
  • Assessment of T-cell reconstitution following BMT via the hepatic portal vein.
  • Analysis of Dll4 and SDF-1 expression in fibrotic liver cells.
  • In vitro studies using Dll4-transfected liver fibroblasts and hematopoietic stem cells (HSCs).
  • Adoptive transfer of T-cell progenitors into Rag-2 deficient hosts.

Main Results:

  • BMT via the hepatic portal vein significantly enhanced T-cell reconstitution in a mouse model of liver fibrosis.
  • Expression of Dll4 (Delta-like 4) was upregulated in hepatocytes of fibrotic livers, induced by TNFα in an NFκB-dependent manner.
  • Liver fibroblasts engineered to express Dll4 (LF-Dll4) promoted T-cell lineage development from HSCs in vitro.
  • Primary liver fibroblasts (primary LF) produced SDF-1, crucial for T-lineage differentiation from HSCs.
  • Generated T-cell progenitors underwent normal maturation upon transfer.

Conclusions:

  • The fibrotic liver microenvironment, through elevated Dll4 and SDF-1, promotes extrathymic T-cell lineage development.
  • These findings offer insights into enhancing T-cell reconstitution under pathological conditions.
  • This research expands understanding of T-cell development and recovery in disease states.