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Sterically stabilized siRNA:gold nanocomplexes enhance c-MYC silencing in a breast cancer cell model
Aliscia N Daniels1, Moganavelli Singh1
1Department of Biochemistry, Nano-Gene & Drug Delivery Laboratory, School of Life Sciences, College of Agriculture, Engineering & Science, University of KwaZulu-Natal (Westville Campus), Private Bag X54001, Durban 4000, Kwa-Zulu Natal, South Africa.
Abstract:
Aim: To produce sterically stabilized and functionalized gold nanoparticles (AuNPs) for efficient delivery of siRNA for c-MYC silencing in vitro. Materials & methods: Synthesized AuNPs were functionalized with chitosan and PEG400 and PEG2000, morphologically and chemically characterized, and assessed for cytotoxicity and gene silencing in vitro. Results & discussion: AuNPs presented as spherical particles in the nanometer size range and successfully bound and protected the siRNA against degradation and were well tolerated in the breast adenocarcinoma (MCF-7) cell line. Nanoparticle-mediated gene knockdown studies revealed enhanced levels of c-MYC gene silencing with more than 90% reduction of MYC protein levels. Conclusion: These nanoformulations show enhanced potential for siRNA-mediated gene silencing in human breast cancer cells in vitro.
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