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Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

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Updated: Jun 8, 2026

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
08:40

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

Challenges in microRNAs' targetome prediction and validation.

Jesus Eduardo Rojo Arias1, Volker Busskamp1

  • 1Center for Regenerative Therapies (CRTD), Technische Universität Dresden, Dresden, Germany.

Neural Regeneration Research
|June 7, 2019
PubMed
Summary

MicroRNAs (miRNAs) are crucial for gene regulation. This review covers advances and challenges in identifying and validating these small RNA molecules, focusing on neuronal miR-124.

Keywords:
miR-124miRNA biogenesismiRNA identificationmiRNA predictionmiRNA regulationmiRNA validationmiRNAswTO analysis

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Last Updated: Jun 8, 2026

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
08:40

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Published on: April 6, 2012

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay
12:49

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Published on: May 25, 2015

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
11:00

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Published on: June 12, 2018

Area of Science:

  • Molecular Biology
  • Genetics
  • Bioinformatics

Background:

  • MicroRNAs (miRNAs) are small RNA molecules regulating gene expression post-transcriptionally.
  • High-throughput sequencing has led to a surge in predicted miRNA candidates, creating data management and validation challenges.
  • Existing miRNA databases require community effort for validating miRNA-target interactions.

Purpose of the Study:

  • To review recent advances and limitations in miRNA prediction, identification, and validation.
  • To highlight the challenges in generating databases of biologically active miRNAs.
  • To discuss the gene regulatory network of miR-124 in human neurons.

Main Methods:

  • Review of current literature on miRNA prediction and validation techniques.
  • Discussion of computational and experimental approaches for miRNA identification.
  • Case study analysis of miR-124's regulatory network in neurons.

Main Results:

  • Significant progress in miRNA prediction technologies, but validation remains a bottleneck.
  • Hurdles in miRNA research include expression patterns, biogenesis, and interaction dynamics.
  • The gene regulatory network of neuronal miR-124 has been elucidated through integrated methods.

Conclusions:

  • Overcoming challenges in miRNA validation is essential for accurate databases.
  • miR-124 plays a significant role in neuronal gene regulation.
  • Combined computational and experimental strategies are effective for understanding complex miRNA networks.