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A split G-quadruplex-specific dinuclear Ir(III) complex for label-free luminescent detection of transcription factor
Menghan Zang1, Huijuan Su1, Lihua Lu1
1College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao 266109, PR China.
Abstract:
A group of luminescent dinuclear Ir(III) complexes were synthesized to study the application possibility as luminescent G-quadruplex (G4) sensors. According to the results, the dinuclear Ir(III) complexes 1-5 were assessed as selective G4 sensors. Among them, complex 5 not only demonstrates the highest specificity towards G4, but also shows linearly enhanced luminescent intensity with the increase of split G4 concentration. Complex 5 was thus utilized to establish a label-free split G4-based detection platform for luminescent detection of transcription factor (TF). This luminescent assay is simple and sensitive with the detection limit of 0.027 nM for NF-κB p50 in aqueous solution. The split G4-based TF sensing platform also worked well in diluted human serum, demonstrating high application potential for biological sample analysis.
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