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Flow cytometric analysis of human bone marrow. II. Normal B lymphocyte development
M R Loken1, V O Shah, K L Dattilio
1Becton Dickinson Monoclonal Center, Mountain View, CA 94043.
Blood
|November 1, 1987
Summary
This study maps B lymphocyte development in human bone marrow using cell surface markers. It reveals a continuous progression of B cell maturation stages defined by antigen expression.
Area of Science:
- Immunology
- Hematology
- Cell Biology
Background:
- B lymphocyte development is a complex process involving sequential changes in cell surface antigen expression.
- Understanding these changes is crucial for diagnosing and treating B cell malignancies and immune deficiencies.
Purpose of the Study:
- To analyze the phenotypic changes during normal human B lymphocyte development in bone marrow.
- To establish a detailed map of B cell maturation stages using multiparameter flow cytometry.
Main Methods:
- Utilized a panel of B lymphoid-reactive monoclonal antibodies for immunophenotyping.
- Employed flow cytometry with quantitative three-color immunofluorescence to analyze cell surface antigens.
- Identified B lymphoid cells using light scattering and CD19 expression.
Main Results:
- CD10 and CD20 expression effectively discriminated between early and mature B lineage cells.
- CD34 further subdivided CD10+ cells, with CD19+, CD10+, CD34+ cells containing the majority of terminal deoxynucleotidyl transferase (TdT+) cells.
- Defined the sequential expression of HLA-DR, HLA-DP, HLA-DQ, IgM, CD20, CD21, and CD22 during B cell development.
Conclusions:
- Multiparameter flow cytometry can define a continuous progression of B lymphocyte development stages.
- Cell surface antigen expression provides a reliable method for mapping B cell maturation, even for minor cell populations.