Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR01:59

CRISPR

57.6K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
57.6K
CRISPR and crRNAs02:53

CRISPR and crRNAs

18.8K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
18.8K
DNA-only Transposons02:57

DNA-only Transposons

17.3K
DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
17.3K
Eukaryotic RNA Polymerases00:58

Eukaryotic RNA Polymerases

26.8K
RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
26.8K
DNA Replication02:40

DNA Replication

58.9K
DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication...
58.9K
RNA Interference01:23

RNA Interference

27.9K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
27.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hawaiian geothermal fumaroles contain diverse and novel viruses.

Microbiology spectrum·2026
Same author

Hawaiian Geothermal Fumaroles Contain Diverse and Novel Viruses.

bioRxiv : the preprint server for biology·2026
Same author

Prokaryotic Schlafen proteins cleave tRNAs during type III CRISPR immunity.

Nature communications·2026
Same author

END nucleases are antiphage defence systems targeting multiple phages with modified genomes.

Nature microbiology·2026
Same author

Ribosomal RNA cleavage by the previously unidentified RelS-RelI toxin-antitoxin system controls growth of Mycobacterium tuberculosis.

Nucleic acids research·2026
Same author

Beyond oncogenesis: the unexplored benefits of viruses in cancer immunity.

Nature reviews. Cancer·2026

Related Experiment Video

Updated: Jan 23, 2026

CRISPR Guide RNA Cloning for Mammalian Systems
06:48

CRISPR Guide RNA Cloning for Mammalian Systems

Published on: October 2, 2018

72.9K

RNA-guided DNA insertion with CRISPR-associated transposases.

Jonathan Strecker1,2,3,4, Alim Ladha1,2,3,4, Zachary Gardner1,2,3,4

  • 1Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.

Science (New York, N.Y.)
|June 8, 2019
PubMed
Summary

Researchers discovered a new CRISPR-Cas system (ShCAST) that precisely inserts DNA into bacterial genomes. This RNA-guided DNA transposition system achieves high efficiency without needing host cell repair, advancing gene editing technologies.

More Related Videos

Substrate Generation for Endonucleases of CRISPR/Cas Systems
11:53

Substrate Generation for Endonucleases of CRISPR/Cas Systems

Published on: September 8, 2012

28.0K
Generation of RNA/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides
16:42

Generation of RNA/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides

Published on: November 24, 2010

27.4K

Related Experiment Videos

Last Updated: Jan 23, 2026

CRISPR Guide RNA Cloning for Mammalian Systems
06:48

CRISPR Guide RNA Cloning for Mammalian Systems

Published on: October 2, 2018

72.9K
Substrate Generation for Endonucleases of CRISPR/Cas Systems
11:53

Substrate Generation for Endonucleases of CRISPR/Cas Systems

Published on: September 8, 2012

28.0K
Generation of RNA/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides
16:42

Generation of RNA/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides

Published on: November 24, 2010

27.4K

Area of Science:

  • Molecular Biology
  • Genetics
  • Microbiology

Background:

  • CRISPR-Cas nucleases are essential for nucleic acid manipulation.
  • Targeted DNA insertion using CRISPR technology faces challenges due to reliance on host cell repair mechanisms.

Purpose of the Study:

  • To characterize a novel CRISPR-associated transposase from cyanobacteria (ShCAST).
  • To evaluate ShCAST's capability for RNA-guided DNA transposition and insertion into bacterial genomes.

Main Methods:

  • Characterization of the ShCAST system, comprising Tn7-like transposase subunits and a Cas12k effector.
  • Assessing ShCAST's DNA insertion efficiency and site specificity in *Escherichia coli*.
  • Investigating the mechanism of RNA-guided DNA transposition.

Main Results:

  • ShCAST catalyzes RNA-guided DNA transposition, inserting DNA segments 60-66 base pairs downstream of the protospacer.
  • ShCAST achieved up to 80% integration efficiency in the *E. coli* genome without positive selection.
  • Demonstrated unidirectional DNA insertion mediated by ShCAST.

Conclusions:

  • ShCAST represents a novel CRISPR-Cas system with transposase activity.
  • This system enables precise, efficient, and selection-independent DNA insertion.
  • Expands understanding of CRISPR-Cas functional diversity and offers a new paradigm for precision genome engineering.