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MiR-876-5p regulates gastric cancer cell proliferation, apoptosis and migration through targeting WNT5A and MITF

Zhenglei Xu1, Zhichao Yu1, Qinghong Tan1

  • 1Department of Gastroenterology, The Second Clinical Medical College (Shenzhen People's Hospital), Jinan University, Shenzhen 518000, Guangdong, China.

Bioscience Reports
|June 8, 2019
PubMed

Insights

MicroRNA-876-5p (miR-876-5p) suppresses gastric cancer (GC) progression by inhibiting proliferation, migration, and epithelial-mesenchymal transition. It targets WNT5A and MITF, revealing a novel mechanism in GC development.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • MicroRNAs (miRNAs) play crucial roles in various cancers, including gastric cancer (GC).
  • The specific molecular mechanisms of many miRNAs in GC remain unclear.
  • miR-876-5p was found to be significantly downregulated in GC cells.

Purpose of the Study:

  • To investigate the functional and mechanistic roles of miR-876-5p in gastric cancer.
  • To elucidate the molecular targets and pathways regulated by miR-876-5p in GC.

Main Methods:

  • Gain-of-function assays to assess miR-876-5p overexpression effects.
  • Bioinformatics prediction and mechanism experiments to identify direct targets.
  • Loss-of-function assays for WNT5A and MITF.
  • Rescue assays to confirm the regulatory pathway.

Main Results:

  • Overexpression of miR-876-5p repressed GC cell proliferation, anti-apoptosis, migration, and epithelial-mesenchymal transition (EMT).
  • miR-876-5p was verified to target both WNT5A and MITF mRNA and protein.
  • WNT5A and MITF were highly expressed in GC cells and exhibited oncogenic roles.
  • miR-876-5p suppressed GC cell viability and migration while inducing apoptosis by targeting WNT5A and MITF.

Conclusions:

  • miR-876-5p acts as a tumor suppressor in gastric cancer.
  • The miR-876-5p/WNT5A/MITF axis represents a novel regulatory pathway in GC.
  • This study provides evidence for the potential therapeutic role of miR-876-5p in GC treatment.
Keywords:
MITFWNT5Agastric cancermiR-876-5p

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