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Author Spotlight: Noninvasive Cerebral Blood Flow Determination in Human Functional Brain Region for Diagnosis of Neurological Disorders
05:23

Author Spotlight: Noninvasive Cerebral Blood Flow Determination in Human Functional Brain Region for Diagnosis of Neurological Disorders

Published on: May 31, 2024

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Influence of different isoflurane anesthesia protocols on murine cerebral hemodynamics measured with

Leon P Munting1,2, Marc P P Derieppe1,3, Ernst Suidgeest1

  • 1Department of Radiology, Leiden University Medical Center, Leiden, the Netherlands.

NMR in Biomedicine
|June 8, 2019
PubMed

Insights

Anesthesia affects mouse brain blood flow (CBF) and reactivity (CVR) measurements. Lower isoflurane concentrations or mechanical ventilation alter CBF and CVR, impacting translational MRI studies.

Area of Science:

  • Neuroimaging
  • Translational Research
  • Anesthesiology

Background:

  • Arterial spin labeling (ASL)-MRI noninvasively measures cerebral blood flow (CBF) and cerebrovascular reactivity (CVR).
  • These parameters are crucial biomarkers for cognitive impairment and dementia research.
  • Mouse models are vital for translational MRI studies, but anesthesia protocols can influence hemodynamic measurements.

Purpose of the Study:

  • To investigate the impact of three distinct isoflurane anesthesia protocols on CBF and CVR in mice.
  • To compare isoflurane effects with a medetomidine anesthesia protocol.
  • To determine the relationship between baseline CBF and CVR under different anesthesia conditions.

Main Methods:

  • Pseudo-continuous ASL-MRI was employed in two mouse strains.
  • Three isoflurane protocols were tested: medium concentration free-breathing, low concentration free-breathing, and medium concentration with mechanical ventilation.
  • A medetomidine anesthesia protocol served as a comparator.

Main Results:

  • Medium isoflurane anesthesia yielded significantly higher CBF and lower CVR compared to medetomidine.
  • Alternative isoflurane protocols (low concentration or mechanical ventilation) resulted in lower CBF and higher CVR than medium concentration isoflurane.
  • Cerebrovascular reactivity (CVR) demonstrated a dependency on baseline cerebral blood flow (CBF) across all tested anesthesia protocols.

Conclusions:

  • Anesthesia protocols significantly influence ASL-MRI derived CBF and CVR in mice.
  • Careful selection and reporting of anesthesia protocols are essential for accurate interpretation of hemodynamic data in translational research.
  • The relationship between CBF and CVR should be considered when analyzing anesthesia-related hemodynamic changes.
Keywords:
anesthesiaarterial spin labeling (ASL)-MRIbrainhemodynamicsisofluranemouse

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