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A "Patient-Like" Orthotopic Syngeneic Mouse Model of Hepatocellular Carcinoma Metastasis
Published on: October 24, 2015
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Tumor-Specific Transcripts Are Frequently Expressed in Hepatocellular Carcinoma With Clinical Implication and
Qiupeng Zheng1,2, Jingjing Zhao1, Hui Yu2,3
1Fudan University Shanghai Cancer Center, Key Laboratory of Medical Epigenetics and Metabolism, Institutes of Biomedical Sciences, Fudan University, Shanghai, China.
Hepatology (Baltimore, Md.)
|June 8, 2019
Summary
Hundreds of tumor-specific transcripts (TSTs) are frequently expressed in hepatocellular carcinoma (HCC), a lethal cancer. These novel noncoding RNAs are linked to poor prognosis and may offer new diagnostic and therapeutic strategies.
Area of Science:
- Genomics
- Oncology
- Molecular Biology
Background:
- Hepatocellular carcinoma (HCC) is a lethal malignancy with complex, understudied etiologies.
- The cancer transcriptome offers potential for discovering tumor-specific transcripts (TSTs).
Purpose of the Study:
- To identify and characterize novel tumor-specific transcripts (TSTs) in hepatocellular carcinoma (HCC).
- To investigate the clinical significance and functional roles of TSTs in HCC.
Main Methods:
- RNA sequencing analysis of approximately 1,000 normal and HCC tissues.
- Assembling spliced junction analysis to identify TSTs.
- Functional screening and analysis of TST regulation and detection.
Main Results:
- Hundreds of frequently expressed TSTs were identified in HCC, many unannotated and noncoding.
- Intergenic TSTs originate from long terminal repeat (LTR) elements.
- TSTs correlate with poor HCC prognosis; a specific noncoding TST (TST1) regulates HCC cell proliferation.
- TST1 is epigenetically regulated and TSTs are detectable in extracellular vesicles.
Conclusions:
- HCC exhibits an abundance of TSTs with significant clinical potential.
- TSTs represent promising targets for novel cancer diagnosis and treatment strategies.
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