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Switchable Enzymatic Accessibility for Precision Cell-Selective Surface Glycan Remodeling.

Peiwen Zhang1, Yiran Li1, Xiaofei Yu1

  • 1State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, P.R. China.

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Summary
This summary is machine-generated.

A novel switchable enzymatic accessibility (SEA) strategy precisely modifies cell surface glycans. This method uses metal-organic framework cages for controlled enzyme access, enabling targeted glycan remodeling with high spatial resolution.

Keywords:
carbohydratescell recognitionglycan remodelingimaging agentsmetal-organic frameworks

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Area of Science:

  • Biochemistry
  • Chemical Biology
  • Materials Science

Background:

  • Cell surface glycan remodeling is crucial for biological processes but lacks precise control.
  • Existing methods struggle with specificity and spatial resolution in complex cellular environments.

Purpose of the Study:

  • To develop a highly specific and spatially controlled method for cell surface glycan remodeling.
  • To demonstrate the utility of switchable enzymatic accessibility (SEA) for precise carbohydrate editing.

Main Methods:

  • Utilized metal-organic framework (MOF) cages to reversibly block enzyme access to cell surfaces.
  • Developed a strategy for instantaneous MOF disassembly to enable enzyme-mediated glycan modification.
  • Applied the SEA strategy for specific remodeling of terminal galactose/N-acetylgalactosamine (Gal/GalNAc) and desialylation of sialic acid (Sia).

Main Results:

  • Achieved precise, cell-selective glycan remodeling using the SEA strategy.
  • Demonstrated high spatial resolving power, distinguishing between immediately neighboring cell lines.
  • Showcased the ability to perform targeted Gal/GalNAc remodeling and Sia desialylation.

Conclusions:

  • The SEA strategy offers a temporally and spatially controlled tool for tuning glycosylation architectures.
  • This modular and adaptable protocol is rapid (ca. 30 min) and highly specific.
  • SEA holds significant potential for applications in tissue-specific imaging, immunotherapy, and fundamental cell biology research.