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Updated: Jan 23, 2026

Protocols of 3D Bioprinting of Gelatin Methacryloyl Hydrogel Based Bioinks
Published on: December 21, 2019
Properties of an alginate-gelatin-based bioink and its potential impact on cell migration, proliferation, and
Liuhanghang Cheng1, Bin Yao2, Tian Hu3
1Department of Plastic Surgery and Burn Center, The Second Affiliated Hospital, Shantou University Medical College, North Dongxia Road, Shantou 515041, Guangdong Province, PR China.
Abstract:
Three-dimensional (3D) bioprinting allows embedding of cells within a bioink, creating cell-based 3D structures to promote tissue regeneration and repair. The bioink should be biocompatible with the cells and its effect on cell behavior should be determined. Alginate-gelatin (Alg-Gel) blends with mouse planta dermis (PD) induced epidermal progenitors for sweat gland regeneration, confirming the role of 3D support during the process. The present study aimed to investigate the chemical and physical properties of the Alg-Gel-PD bioink, and its effect on embedded mouse mesenchymal stem cells (MSCs). MSCs showed increased proliferation and migration in 2D culture with an Alg-Gel-based bioink extract. Gene expression analysis confirmed MSC differentiation towards sweat gland cells. The extract had no effect on protein expression in differentiated cells. Mixing MSCs with the Alg-Gel-based bioink for 3D bioprinting resulted in gene and protein expression characteristic of differentiation, including YAP1 activation. The mechanical strength of the bioink was similar to that of mouse dermal tissue and scanning electron microscopy showed that PD induced a more regular pore structure, suggesting advantages for the physical properties of the embedded cells. This study determined the influence of bioink on cellular behavior, thereby promoting therapeutic stem cell function via 3D cell printing processes.
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