Related Experiment Video
Updated: Jan 23, 2026

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope
Published on: October 9, 2014
Pyrene Based Fluorescent Turn-on Chemosensor for Sequential Detection of Fe3+ and Fe2+ Ions and its Application in
Rajasekaran Dhivya1, Asaithambi Gomathi1, Periasamy Viswanathamurthi2
1Department of Chemistry, Periyar University, Salem, 636 011, India.
Abstract:
A simple pyrene-based "turn-on" chemosensor bearing isonizide namely 3-(pyren-1-yl methylene) pentane-2,4-diyldene di(isonicotinohydrazide) (PMPD) was designed and synthesized for detecting Fe3+and Fe2+ ions in HEPES buffer solution at pH 7.4 (DMSO: H2O (1:9 v/v)) medium. The probe shows conducive selectivity for Fe2+ and Fe3+ ions over the other competitive metal cations. The detection limit was found to be 1.67 μM for Fe3+ and 2.02 μM for Fe2+. The recognition mechanism of PMPD towards Fe3+ and Fe2+ has been examined in detail by absorption, emission, and ESI-MS studies. Moreover, "turn-on" fluorescence behavior of the probe was used to track iron ions in living cells.
Related Concept Videos
Ions as Acids and Bases
Salts are ionic compounds composed of cations and anions, either of which may be capable of undergoing an acid or base ionization reaction with water. Aqueous salt solutions, therefore, may be acidic, basic, or neutral, depending on the relative acid-base strengths of the salt’s constituent ions. For example, dissolving the ammonium chloride in water results in its dissociation, as described by the equation:
Common Ion Effect
Ion Channels
Ion channels are specialized integral membrane proteins on the plasma membrane that allow...
Formation of Complex Ions
Precipitation of Ions
The equation that describes the equilibrium between solid calcium carbonate and its solvated ions is:
Ions and Ionic Charges

