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Long-read nanopore sequencing-based draft genome of a carbapenem-resistant Pseudomonas aeruginosa isolate

Xiaoling Yu1, Zhaofang Han2, Chengsong Ye3

  • 1Infectious Diseases Department, Mengchao Hepatobiliary Hospital of Fujian Medical University, 312 Xihong Road, Gulou District, Fuzhou, 350025, Fujian, China.

Journal of Global Antimicrobial Resistance
|June 9, 2019
PubMed
Abstract

Insights

We sequenced the genome of a carbapenem-resistant Pseudomonas aeruginosa (CRPA) isolate. This research identifies specific gene mutations responsible for carbapenem resistance in this common pathogen.

Area of Science:

  • Microbiology
  • Genomics
  • Infectious Diseases

Background:

  • Pseudomonas aeruginosa is a Gram-negative bacterium responsible for severe infections, particularly in catheterized patients.
  • Carbapenem resistance is a growing concern in managing P. aeruginosa infections.

Purpose of the Study:

  • To report the draft genome sequence of a carbapenem-resistant P. aeruginosa (CRPA) isolate.
  • To identify genetic determinants of carbapenem resistance.

Main Methods:

  • Whole-genome sequencing utilizing a hybrid approach with Illumina short reads and Oxford Nanopore long reads.
  • Bioinformatic analysis for genome assembly and gene annotation.

Main Results:

  • A contiguous genome assembly of 6,624,003 bp with 66.21% GC content was generated.
  • 6,389 protein-coding genes were identified.
  • Mutations in the oprD and mexR genes were found to be associated with carbapenem resistance.

Conclusions:

  • The draft genome sequence provides a foundation for studying CRPA resistance mechanisms.
  • This data can aid in developing novel therapeutic strategies against drug-resistant P. aeruginosa.
Keywords:
Carbapenem resistanceGenomeNanopore sequencingPseudomonas aeruginosa

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