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Updated: Jan 23, 2026

In Vitro Assays to Evaluate the Migration, Invasion, and Proliferation of Immortalized Human First-trimester Trophoblast Cell Lines
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In Vitro Assays to Evaluate the Migration, Invasion, and Proliferation of Immortalized Human First-trimester Trophoblast Cell Lines

Published on: March 5, 2019

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Formyl-peptide receptor 2 suppresses proliferation, migration and invasion in human extravillous trophoblastic cells

Shenzhi Zhao1, Tingting Liao2, Tong Zhou2

  • 1The Second Clinical College, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325027, People's Republic of China.

Prostaglandins & Other Lipid Mediators
|June 10, 2019
PubMed

Insights

Overexpressing the FPR2 receptor in human extravillous trophoblast (TEV-1) cells inhibited proliferation, migration, and invasion. This suggests ideal FPR2 levels are crucial for normal TEV-1 cell function.

Area of Science:

  • Reproductive biology
  • Cell biology
  • Molecular signaling

Background:

  • The function of the Formyl Peptide Receptor 2 (FPR2) in human extravillous trophoblast (TEV-1) cells is not well understood.
  • FPR2 is known to be present in the endometrium and placenta.

Purpose of the Study:

  • To investigate the role of FPR2 in regulating the proliferation, migration, and invasion of human extravillous trophoblast (TEV-1) cells.
  • To elucidate the molecular mechanisms underlying FPR2's function in TEV-1 cells.

Main Methods:

  • Overexpression of FPR2 in TEV-1 cells.
  • Cell proliferation was assessed using CCK8 assays.
  • Cell migration and invasion were evaluated using transwell and wound healing assays.
  • mRNA and protein levels of key signaling molecules were measured.

Main Results:

  • FPR2 overexpression significantly inhibited TEV-1 cell proliferation, migration, and invasion.
  • FPR2 overexpression led to decreased expression of integrin-linked kinase (ILK), nuclear factor-kappa B (NF-κB), matrix metalloproteinase 9 (MMP9), and vascular endothelial growth factor (VEGF).
  • These effects were mediated through the ILK/NF-κB signaling pathway.

Conclusions:

  • FPR2 plays a critical role in regulating the functional properties of human extravillous trophoblast (TEV-1) cells.
  • Maintaining optimal FPR2 levels is essential for proper TEV-1 cell function during placental development.
  • The ILK/NF-κB signaling pathway is implicated in the mechanism by which FPR2 influences TEV-1 cell behavior.
Keywords:
Extravillous trophoblastic cellsFormyl peptide receptor-2Lipoxin A4

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