Related Experiment Video

Updated: Jan 23, 2026

Treatment with Vancomycin Loaded Calcium Sulphate and Autogenous Bone in an Improved Rabbit Model of Bone Infection
09:09

Treatment with Vancomycin Loaded Calcium Sulphate and Autogenous Bone in an Improved Rabbit Model of Bone Infection

Published on: March 14, 2019

9.4K

A rapid and simple UPLC method for serum vancomycin determination in pediatric patients undergoing continuous

Min Zhang1, XiaoLan Huang2, Wu Yahui3

  • 1Beijing Municipal Key Laboratory of Child Development and Nutriomics, Capital Institute of Pediatrics, Beijing, 100020, China.

Journal of Pharmaceutical and Biomedical Analysis
|June 10, 2019
PubMed

Insights

A new UPLC method accurately measures vancomycin levels in children. This supports therapeutic drug monitoring for both continuous and intermittent vancomycin infusions in pediatric patients.

Area of Science:

  • Pharmacology
  • Analytical Chemistry
  • Pediatric Medicine

Background:

  • Vancomycin is crucial for Gram-positive bacterial infections, including MRSA.
  • Continuous vancomycin infusion shows benefits in adults, but optimal pediatric dosing remains unclear.
  • Accurate therapeutic drug monitoring is essential for effective vancomycin use in children.

Purpose of the Study:

  • To develop and validate a simple, cost-effective UPLC method for pediatric vancomycin serum level determination.
  • To apply this method for therapeutic drug monitoring in children receiving continuous versus intermittent vancomycin infusions.

Main Methods:

  • Utilized Ultra-Performance Liquid Chromatography (UPLC) with protein precipitation and filtration.
  • Method validated for linearity (5-100 mg/L, R²=0.999), precision, and accuracy.
  • Assessed serum vancomycin stability at room temperature and after freeze-thaw cycles.

Main Results:

  • The UPLC method is rapid (5 min/sample), precise (intraday %CV 1.23-6.01%, interday %CV 3.02-9.75%), and accurate (91.21-112.63%).
  • Serum vancomycin demonstrated stability for 12 hours at room temperature and through 3 freeze-thaw cycles.
  • Processed samples remained stable in the autosampler for 15.5 hours.

Conclusions:

  • A validated, simple, and cost-effective UPLC assay is suitable for pediatric vancomycin therapeutic drug monitoring.
  • This method facilitates comparison of continuous versus intermittent vancomycin infusion strategies in children.
  • The validated assay supports optimized vancomycin dosing and improved patient outcomes in pediatric care.

Keywords:
Therapeutic drug monitoringUPLCVancomycincontinuous infusionintermittent infusion

More Related Videos

Author Spotlight: Exploring Venous Waveforms for Non-Invasive Respiratory Monitoring in Pigs04:10

Author Spotlight: Exploring Venous Waveforms for Non-Invasive Respiratory Monitoring in Pigs

Published on: March 8, 2024

1.8K
Pancreatic Duct Infusion: An Effective and Selective Method of Drug and Viral Delivery06:03

Pancreatic Duct Infusion: An Effective and Selective Method of Drug and Viral Delivery

Published on: September 30, 2021

5.5K

Related Concept Videos

Drug Accumulation During Multiple Dosing: Intermittent IV Infusions01:24

Drug Accumulation During Multiple Dosing: Intermittent IV Infusions

Intermittent intravenous (IV) infusion is a method of drug administration where medications are delivered over short infusion periods followed by intervals of no drug delivery. This approach helps to prevent sustained high drug concentrations in the bloodstream, reducing the risk of adverse effects associated with prolonged exposure. Unlike continuous infusion, steady-state concentrations may not be achieved during a single dosing cycle but can be reached through repeated...
240
One-Compartment Model: IV Infusion01:09

One-Compartment Model: IV Infusion

Intravenous (IV) infusion is often utilized when continuous and controlled drug delivery is necessary, such as during surgery or in the treatment of chronic diseases. This method offers numerous advantages, including immediate drug action, precise control over dosage, and bypassing the first-pass metabolism.
The one-compartment model for IV infusion uses mathematical equations to describe the rate of change in drug quantity in the body. At steady-state or infusion equilibrium, the drug input...
502
Two-Compartment Open Model: IV Infusion01:15

Two-Compartment Open Model: IV Infusion

A two-compartment model is a vital tool in pharmacokinetics, providing an essential understanding of drug behavior, especially for those administered via zero-order intravenous infusion. This model outlines two compartments: the central compartment, where elimination occurs, and the peripheral compartment.
The model illustrates the decrease in plasma drug concentration from the central compartment with a specific equation. It shows that under steady-state conditions, the drug's input rate...
575
Pharmacokinetics in Pediatric Patients: Drug Excretion01:26

Pharmacokinetics in Pediatric Patients: Drug Excretion

In pediatric medicine, understanding the renal function and drug elimination nuances is crucial for administering safe and effective treatments. Newborns, in particular, display markedly slower renal functions than adults, profoundly affecting how drugs are cleared from their bodies. This slower drug clearance requires clinicians to extend the dosing intervals for many medications to prevent drug accumulation and toxicity while ensuring therapeutic efficacy.One key area where these adjustments...
214
Pharmacokinetics in Pediatric Patients: Drug Distribution01:17

Pharmacokinetics in Pediatric Patients: Drug Distribution

Drug distribution in the pediatric population exhibits unique challenges and considerations due to the physiological differences between children, particularly neonates and infants, and adults. A crucial aspect of pediatric pharmacology is understanding how these differences impact the pharmacokinetics of various drugs, necessitating age-specific dosing strategies to ensure efficacy and safety.Neonates and infants have a higher total body water content, ~75%–90% of their body weight,...
271
Pharmacokinetics in Pediatric Patients: Drug Metabolism01:24

Pharmacokinetics in Pediatric Patients: Drug Metabolism

In pediatric care, understanding the nuances of hepatic drug metabolism is crucial, as it significantly differs from that of adults. This divergence is primarily due to the developmental stage of drug-metabolizing enzymes, which affects how medications are processed in the body. In neonates, for instance, the activity of Phase I enzymes—critical for the initial breakdown of drugs—is markedly reduced, functioning at just 20–40% of the levels seen in adults. This reduction poses...
199