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Simple, rapid method for converting a peptide from one salt form to another.

T F Gabriel1

  • 1Peptide Research Department, Roche Research Center, Hoffmann-La Roche Inc., Nutley, New Jersey.

International Journal of Peptide and Protein Research
|July 1, 1987
PubMed
Summary

A new method converts peptide trifluoroacetate salts to acetate salts using reversed-phase HPLC. This process, demonstrated with Growth Hormone-Release Factor, achieves high recovery and avoids product degradation, offering broad applicability for peptide salt conversion.

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Peptide Chemistry

Background:

  • Peptide trifluoroacetate salts are common but can interfere with downstream applications.
  • Conversion to acetate salts is often desirable for improved peptide characterization and biological activity.

Purpose of the Study:

  • To develop a robust method for converting peptide trifluoroacetate salts to acetate salts.
  • To ensure the conversion process does not degrade the peptide or leave residual trifluoroacetate.

Main Methods:

  • Utilized reversed-phase High-Performance Liquid Chromatography (RP-HPLC).
  • Employed a volatile buffer system compatible with RP-HPLC.
  • Applied the method to Growth Hormone-Release Factor (GRF(1-44)-NH2) trifluoroacetate.

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Main Results:

  • Achieved greater than 95% recovery of the peptide acetate salt.
  • Extensive analytical studies confirmed the complete absence of trifluoroacetate.
  • Verified that the peptide remained undegraded throughout the conversion process.

Conclusions:

  • The developed RP-HPLC method provides an effective means for converting peptide trifluoroacetate salts to acetate salts.
  • The procedure is highly efficient, preserves peptide integrity, and is expected to be broadly applicable to various peptides and salt conversions.