Related Experiment Video
Updated: Jan 23, 2026

Symptom Assessment of Patients with Allergic Rhinitis Using an Allergen Exposure Chamber
Published on: March 3, 2023
Development of a Dipstick Assay for Soy Allergens: Inexpensive Detection to Control Allergen Exposure
Daniel Álvarez-Simón1, Susana Gómez-Ollés2,3
1Laboratori de Pneumologia, Institut de Recerca Vall d'Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain.
Abstract:
Allergen measurements are use in the food industry and are also routinely performed as part of indoor air quality investigations and occupational health monitoring. In this chapter we describe how to develop a simple, convenient, rapid test to analyze soy allergens that can be used in production environments by non-skilled staff to facilitate immediate corrective action and minimize risk and that can be produced in laboratories not equipped with sophisticated instruments.The strip assay described consists of a membrane that is bonded to an adhesive support where an absorbent pad is also attached to absorb excess reagents. The membrane is stripped with specific antibodies against soy allergens in this case (test line) and a goat anti-rabbit IgG antibody (control line). The strip is exposed to a mix of sample and gold-conjugated specific antibody. Colored bands are read out visually or by densitometry.
Related Concept Videos
Allergic Reactions
Control System Problem
When forming a closed-loop system, issues can arise if the poles cross into the unstable region, leading to potential...
Types of Biopharmaceutical Studies: Controlled and Non-Controlled Approaches
Non-controlled studies, commonly employed for initial exploration, lack a control group, rendering them susceptible to biases and external influences. In contrast,...
Sustainable Development
Combinatorial Gene Control
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
Control Systems
At the heart...

