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Related Experiment Videos

Pseudocoagulase activity of staphylococci.

Z Wegrzynowicz, P B Heczko, J Jeljaszewicz

    Journal of Clinical Microbiology
    |January 1, 1979
    PubMed
    Summary

    Staphylocoagulase activity in human infection strains was assessed. Proteolytic enzymes in staphylococci can interfere with accurate staphylocoagulase testing, necessitating enzyme inhibitors for reliable results.

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    [Prevention of infection with Streptococcus pneumoniae].

    Medizinische Klinik (Munich, Germany : 1983)·2001

    Area of Science:

    • Microbiology
    • Clinical Diagnostics

    Background:

    • Staphylocoagulase is a key virulence factor in Staphylococcus species.
    • Accurate detection of staphylocoagulase is crucial for identifying pathogenic strains.

    Purpose of the Study:

    • To evaluate staphylocoagulase activity in clinical isolates.
    • To investigate the interference of bacterial proteolytic enzymes in staphylocoagulase assays.

    Main Methods:

    • Tested 245 staphylococci strains from human infections for coagulase activity.
    • Utilized rabbit plasma with and without thrombin/proteolytic enzyme inhibitors (Trasylol, heparin, hirudin).
    • Assessed both bacterial suspensions and cell-free supernatants.

    Main Results:

    • 237 strains clotted normal plasma.
    • Inhibitor addition affected results: 222 with heparin, 173 with hirudin.
    • Proteolytic enzymes likely interfere with staphylocoagulase-induced clotting.

    Conclusions:

    • Proteolytic enzymes in staphylococci can lead to false-positive coagulase tests.
    • Incorporating proteolytic enzyme inhibitors enhances the accuracy of staphylocoagulase detection.
    • Refined diagnostic methods are needed to avoid misidentification of staphylococcal strains.

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