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Introducing an Experimental Design Approach for Efficient Optimization of Chiral Derivatization Conditions for D- and
Takahiro Takayama1, Hajime Mizuno1, Toshimasa Toyo'oka1
1Laboratory of Analytical and Bio-Analytical Chemistry, School of Pharmaceutical Sciences, University of Shizuoka.
Abstract:
A sensitive analytical method was developed for individual analyses of D- and L-glyceric acids by chiral derivatization - liquid chromatography-tandem mass spectrometry. To elucidate rapid and efficient optimization for derivatization we newly introduced a concept of design of experiments (DOE). The optimization of major 5 factors in the derivatization could be predicted with only 28 measurements. By applying DOE to optimization, the yields of desired derivatives increased five-fold against before optimization.
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