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Related Experiment Video

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Aptameric Probe Specifically Binding Protein Heterodimer Rather Than Monomers.

Tao Bing1,2, Luyao Shen1,2, Junyan Wang1,2

  • 1Beijing National Laboratory for Molecular Sciences Key Laboratory of Analytical Chemistry for Living Biosystems CAS Research/Education Center for Excellence in Molecular Sciences Institute of Chemistry Chinese Academy of Sciences Beijing 100190 China.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|June 11, 2019
PubMed
Summary

Researchers developed a novel DNA aptamer (BG2) for detecting alkaline phosphatase (AP) heterodimers, not monomers, in cells and tissues. This molecular probe enables in situ visualization and isolation of AP heterodimers, advancing protein dimer research.

Keywords:
alkaline phosphataseaptamersmolecular probesprotein heterodimersrecognition

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biotechnology

Background:

  • Protein dimerization is crucial for biological functions.
  • Lack of specific molecular probes hinders in situ detection of protein dimers.
  • Studying protein dimers, like alkaline phosphatase (AP) heterodimers, is challenging.

Purpose of the Study:

  • To develop a specific molecular probe for detecting alkaline phosphatase (AP) heterodimers.
  • To utilize the probe for in situ analysis and isolation of AP heterodimers.
  • To explore the potential of the probe in cancer research and diagnostics.

Main Methods:

  • Systematic evolution of ligands by exponential enrichment (cell-SELEX) to generate a G-rich DNA aptamer (BG2).
  • Characterization of BG2's binding specificity to AP heterodimers versus monomers.
  • Application of BG2 as a molecular probe for cell imaging and affinity isolation.

Main Results:

  • BG2 selectively binds to AP heterodimers, folding into a G-quadruplex structure.
  • AP heterodimers are detected on various cancer cells.
  • BG2 successfully isolates AP heterodimers from cell lysates and enables tumor imaging in vivo.

Conclusions:

  • BG2 is a potent molecular probe for specific detection and isolation of AP heterodimers.
  • The development of BG2 opens new avenues for studying the function and distribution of AP heterodimers.
  • Cell-SELEX-generated aptamers offer a promising approach for protein dimer research and biomedical applications.