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Updated: Jan 27, 2026

Co-Culture and Transduction of Murine Thymocytes on Delta-Like 4-Expressing Stromal Cells to Study Oncogenes in T-Cell Leukemia
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Co-Culture and Transduction of Murine Thymocytes on Delta-Like 4-Expressing Stromal Cells to Study Oncogenes in T-Cell Leukemia

Published on: June 9, 2023

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[Demonstration of the oncogenic activity of cell cultures using antithymocytic serum]

C Abel, H Glathe

    Journal of Hygiene, Epidemiology, Microbiology, and Immunology
    |January 1, 1978
    PubMed

    Insights

    Antithymocytic serum (ATC) effects on mice varied by cell strain, influencing tumor development and growth. Calf-derived ATC serum showed lower toxicity compared to rabbit-derived serum.

    Area of Science:

    • Immunology
    • Oncology
    • Cell Biology

    Background:

    • Antithymocytic serum (ATC) is used to modulate the immune system.
    • The effect of different cell strains on tumor development in mice treated with ATC serum requires investigation.
    • Understanding the comparative toxicity of different sources of ATC serum is important for preclinical studies.

    Purpose of the Study:

    • To evaluate the impact of 10 different cell strains on tumor development in mice treated with antithymocytic serum (ATC).
    • To compare the toxicity of calf-derived versus rabbit-derived ATC serum in mice.
    • To identify cell strains that do not induce tumor formation under ATC serum treatment.

    Main Methods:

    • Administration of antithymocytic serum (ATC) from calf and rabbit sources to mice.
    • Inoculation of mice with 10 different cell strains of varying origins.
    • Observation and recording of tumor development, number, and growth tendency.
    • Assessment of the toxicity of different ATC serum sources.

    Main Results:

    • Tumor development and growth tendency varied significantly depending on the cell strain used.
    • Diploid cell strains WI 38 and LEP, along with primary chicken fibroblast cultures, did not result in tumor formation.
    • Control mice (without ATC serum) occasionally developed small, transient ganglions.
    • Calf-derived ATC serum exhibited lower toxicity in mice compared to rabbit-derived ATC serum.

    Conclusions:

    • The choice of cell strain is critical when studying tumor development in mice treated with antithymocytic serum (ATC).
    • Certain cell strains, including WI 38, LEP, and chicken fibroblasts, appear safe for use in conjunction with ATC serum regarding tumor induction.
    • Calf-derived ATC serum is a potentially safer alternative to rabbit-derived serum due to its reduced toxicity in mice.

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