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Published on: December 13, 2017
Steroidomics for highlighting novel serum biomarkers of testosterone doping
Federico Ponzetto1,2, Julien Boccard3,4, Raul Nicoli1
1Swiss Laboratory for Doping Analyses, University Center of Legal Medicine Geneva & Lausanne, Centre Hospitalier Universitaire Vaudois & University of Lausanne, 1066 Epalinges, Switzerland.
Abstract:
Aim: Quantification of testosterone (T) and 5α-dihydrotestosterone serum concentrations proved to be an efficient alternative to urinary steroid profiling for the detection of T doping. In this context, additional serum markers could be discovered by exploratory untargeted steroidomics studies. Results: Endogenous steroid metabolites were monitored by ultra high-performance liquid chromatography coupled to high-resolution mass spectrometry in serum samples collected during a T administration clinical trial. A three-step workflow for accurate review of annotation was used and multifactorial data analysis allowed highlighting promising serum biomarkers. Longitudinal monitoring of selected compounds was performed to assess T abuse detection capabilities. Conclusion: Application of serum steroidomics showed high potential for biomarker discovery of T doping, suggesting longitudinal monitoring of steroid hormones in serum as a significant improvement in detection of endogenous steroids abuse.
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